Potential antibacterial activity of partial purified bromelain from pineapple core extracts using acetone and ammonium sulphate against dental caries-causing bacteria

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Abstract

This study was conducted to isolate and purify bromelain from pineapple core (Ananas comosus (L.) Merr.) by multisteps fractionation using acetone and ammonium sulphate. The antibacterial activity of bromelain fraction with the highest specific activity was examined using disc diffusion method against S. mutans and P. gingivalis, bacteria that cause dental caries and periodontal disease. The crude enzyme showed proteolitic activity with the specific activity of 52.318 Unit/mg. Fractionation of crude enzyme using acetone produced the third fraction with level of saturation of 50-80 % that has a highest specific activity of 87.778 Unit/mg. Further purification by fractionation using ammonium sulphate produced the third fraction which has a specific activity value increase to 260 Unit/mg. After dialysis, the specific activity of the ammonium sulphate third fraction was increased to 340.926 Unit/mg. The antibacterial assay showed that ammonium sulphate fraction has a strong activity against P. gingivalis bacteria with diameter of clear zone of 21 mm and weak activity against S. mutans with diameter of clear zone of 12 mm.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Ananas comosus
  • P. gingivalis
  • bromelain
  • multilevels fractionation
  • pineapple core

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    Amini, N., Setiasih, S., Handayani, S., Pws, S. H., & Saefudin, E. (2018). Potential antibacterial activity of partial purified bromelain from pineapple core extracts using acetone and ammonium sulphate against dental caries-causing bacteria. In R. Yuniati, T. Mart, I. T. Anggraningrum, D. Triyono, & K. A. Sugeng (Eds.), Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 [020071] (AIP Conference Proceedings; Vol. 2023). American Institute of Physics Inc.. https://doi.org/10.1063/1.5064068