Potency of growth promoting bacteria on mycelial growth of edible mushroom Pleurotus ostreatus and its identification based on 16S rDNA analysis

Evan Febriansyah, Iwan Saskiawan, Wibowo Mangunwardoyo, Tri Ratna Sulistiyani, Eva Watamtin Widhiya

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

The oyster mushroom (Pleurotus ostreatus) is one of the most widely cultivated mushrooms in the world. Nutrients provided in the media is one of the most important factors affecting the growth of mushroom. The present study mainly focuses on the isolation of Mushroom Growth Promoting (MGP) bacteria and studies its effect on the mycelial growth. Nineteen bacteria were isolated from the media of Pleurotus ostreatus and their effect on mycelial growth of the Pleurotus ostreatus was investigated. Based on mycelial growth on solid medium shows difference among treatments in the day taken for the mycelium to cover the petri dish (three days earlier than control). Three bacteria isolate with the best result were identified based on 16S rDNA sequencing and phylogenetic tree construction by the neighbor-joining method using MEGA version 6.06 based on 1 000 × bootstraps and identified as Bacillus cereus strain ATCC 14579, Bacillus aryabhattai strain B8W22 and Acinetobacter pittii strain ATCC 19004. The results of this research strongly suggest that inoculation of the mycelium with specific bacteria supernatant may have beneficial applications for mushroom production.

Original languageEnglish
Title of host publicationInventing Prosperous Future through Biological Research and Tropical Biodiversity Management
Subtitle of host publicationProceedings of the 5th International Conference on Biological Science
EditorsZuliyati Rohmah, Lisna Hidayati, Tuty Arisuryanti, Maryani, Ganies Riza Aristya
PublisherAmerican Institute of Physics Inc.
ISBN (Print)9780735417182
DOIs
Publication statusPublished - 15 Aug 2018
Event5th International Conference on Biological Science: Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management, ICBS 2017 - Yogyakarta, Indonesia
Duration: 15 Sep 201716 Sep 2017

Publication series

NameAIP Conference Proceedings
Volume2002
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Conference on Biological Science: Inventing Prosperous Future through Biological Research and Tropical Biodiversity Management, ICBS 2017
CountryIndonesia
CityYogyakarta
Period15/09/1716/09/17

Keywords

  • MGP
  • Pleurotus ostreatus
  • bacteria

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