TY - JOUR
T1 - Possible role of Porphyromonas gingivalis in the regulation of E2F1, CDK11, and iNOS gene expression in neuronal cell cycle
T2 - A preliminary study
AU - Bachtiar, Endang
AU - Septiwidyati, Tienneke
N1 - Funding Information:
This study was supported by the research grant from Universitas Indonesia PUTI Saintekes (NKB-4811/ UN2.RST/HKP.05.00/2020).
Publisher Copyright:
© 2021 Journal of the Anatomical Society of India. All rights reserved.
PY - 2021/9/1
Y1 - 2021/9/1
N2 - Objective: This study aimed at evaluating the in vitro effect of Porphyromonas gingivalis exposure in gene expression of E2F1 (family of transcription factors), cyclin-dependent kinase-1 (CDK11), and inducible nitric oxide synthase (iNOS) of the neuronal cell cycle. Materials and Methods: The culture of neuronal cell line SH-SY5Y was exposed to P. gingivalis ATCC 33277, and the gene expression of E2F1, CDK11, and iNOS was analyzed by using a real-time polymerase chain reaction. Results: It was shown that E2F1, a G1 phase biomarker and transcription factor, was upregulated in neuronal cells exposed to P. gingivalis compared with that in control cells. However, CDK11, a biomarker of G2/M checkpoint and iNOS, was downregulated in neuronal cells exposed to P. gingivalis compared with that in control cells. Conclusions: P. gingivalis can regulate the neuronal cell cycle, as indicated in the E2F1, CDK11, and iNOS gene expression.
AB - Objective: This study aimed at evaluating the in vitro effect of Porphyromonas gingivalis exposure in gene expression of E2F1 (family of transcription factors), cyclin-dependent kinase-1 (CDK11), and inducible nitric oxide synthase (iNOS) of the neuronal cell cycle. Materials and Methods: The culture of neuronal cell line SH-SY5Y was exposed to P. gingivalis ATCC 33277, and the gene expression of E2F1, CDK11, and iNOS was analyzed by using a real-time polymerase chain reaction. Results: It was shown that E2F1, a G1 phase biomarker and transcription factor, was upregulated in neuronal cells exposed to P. gingivalis compared with that in control cells. However, CDK11, a biomarker of G2/M checkpoint and iNOS, was downregulated in neuronal cells exposed to P. gingivalis compared with that in control cells. Conclusions: P. gingivalis can regulate the neuronal cell cycle, as indicated in the E2F1, CDK11, and iNOS gene expression.
KW - CDK11
KW - E2F1
KW - iNOS
KW - neuron
KW - Porphyromonas gingivalis
UR - http://www.scopus.com/inward/record.url?scp=85116237583&partnerID=8YFLogxK
U2 - 10.4103/jispcd.JISPCD_108_21
DO - 10.4103/jispcd.JISPCD_108_21
M3 - Article
AN - SCOPUS:85116237583
SN - 2231-0762
VL - 11
SP - 582
EP - 587
JO - Journal of International Society of Preventive and Community Dentistry
JF - Journal of International Society of Preventive and Community Dentistry
IS - 5
ER -