TY - JOUR
T1 - Possible intraindividual evolution of SARS-CoV-2 in nasopharyngeal and anal swab in an octogenarian
T2 - a case report
AU - Zaini, Jamal
AU - Putra, Andika Chandra
AU - Ridwanuloh, Asep Muhamad
AU - Saniyyah, Zahrah
AU - Haryanto, Budi
AU - Utomo, Ahmad Rusdan Handoyo
AU - Dharmayanthi, Anik Budhi
AU - Prasetyoputri, Anggia
AU - Andriani, Ade
AU - Hariyatun, Hariyatun
AU - Nuryana, Isa
AU - Iryanto, Syam Budi
AU - Saputra, Sugiyono
AU - Wardiana, Andri
AU - Ningrum, Ratih Asmana
N1 - Funding Information:
The authors would like to thank to COVID-19 Detection Team from BSL-3 Laboratory, Research Organization for Life Sciences, National Research and Innovation Agency, Republic of Indonesia, Cibinong.
Publisher Copyright:
© GERMS 2022.
PY - 2022
Y1 - 2022
N2 - Introduction COVID-19 is an emerging infectious disease that remains to be further investigated. Case report Here, we describe a case of COVID-19 in an octogenarian woman with comorbidities who slowly recovered during hospitalization, but died due to sudden cardiac death after 2 weeks of hospitalization. Her nasopharyngeal and anal swabs returned positive for SARS-CoV-2 by RT-PCR on day 7 of hospitalization. The NGS showed possible intraindividual evolution of virus. The sample from the nasopharyngeal swab yielded a B.1470 variant classified as clade GH. This variant showed mutation in the spike gene D614G; N gene; NS3 gene; NSP2 gene and NSP12 gene. The sample from the anal swab showed similar mutation but with additional point mutation in spike gene S12F and was classified as B.1.465 variant. Conclusions The possibility of the gastrointestinal tract that served as reservoir for virus mutation accumulation should also be considered and the potential impact of viral fecal transmission in the environment should be further investigated.
AB - Introduction COVID-19 is an emerging infectious disease that remains to be further investigated. Case report Here, we describe a case of COVID-19 in an octogenarian woman with comorbidities who slowly recovered during hospitalization, but died due to sudden cardiac death after 2 weeks of hospitalization. Her nasopharyngeal and anal swabs returned positive for SARS-CoV-2 by RT-PCR on day 7 of hospitalization. The NGS showed possible intraindividual evolution of virus. The sample from the nasopharyngeal swab yielded a B.1470 variant classified as clade GH. This variant showed mutation in the spike gene D614G; N gene; NS3 gene; NSP2 gene and NSP12 gene. The sample from the anal swab showed similar mutation but with additional point mutation in spike gene S12F and was classified as B.1.465 variant. Conclusions The possibility of the gastrointestinal tract that served as reservoir for virus mutation accumulation should also be considered and the potential impact of viral fecal transmission in the environment should be further investigated.
KW - anal swab
KW - evolution
KW - octogenarian
KW - SARS-CoV-2
UR - http://www.scopus.com/inward/record.url?scp=85134410034&partnerID=8YFLogxK
U2 - 10.18683/germs.2022.1332
DO - 10.18683/germs.2022.1332
M3 - Article
AN - SCOPUS:85134410034
SN - 2248-2997
VL - 12
SP - 298
EP - 303
JO - GERMS
JF - GERMS
IS - 2
ER -
