Polyol production by culture of methanol-utilizing yeast

Herman Suryadi, Tohoru Katsuragi, Nobuyuki Yoshida, Shinsuke Suzuki, Yoshiki Tani

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Four methanol-utilizing yeasts, Candida boidinii, Hansenuia polymorpha. Hansenula ofunaensis, and Pichia pinus, produced polyols from corresponding sugars in a methanol medium. H. polymorpha produced larger amounts of xylitol than the other yeasts. Productivity was the highest at pH 8 when 5 g (dry)/I cultured cells were incubated with 2.5 g/l urea as the nitrogen source in a medium containing 1% (v/v) methanol and 1 g/l MgSO4 · 7H2O. Under these conditions, 57 g/l xylitol was obtained from 110 g/l p-xylose after 3 d of cultivation. The largest amount of xylitol (58 g/l; yield, 0.62 g/g) was produced from 125 g/l D-xylose and 5% (w/v) glycerol instead of methanol after 4 d of cultivation.

Original languageEnglish
Pages (from-to)236-240
Number of pages5
JournalJournal of Bioscience and Bioengineering
Issue number3
Publication statusPublished - 2000


  • Glycerol
  • Hansenula polymorpha
  • Methanol yeast
  • Polyol
  • Xylitol
  • Xylose


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