Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population

Nisa Ajeng Puspitasari; Yurnadi Hanafi Midoen; Nurtami Soedarsono; Niniarty Djamal; Triana Marchelina; Elza Ibrahim Auerkari

doi:10.1063/1.5096755

Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population

Nisa Ajeng Puspitasari
, Yurnadi Hanafi Midoen
, Nurtami Soedarsono
, Niniarty Djamal
, Triana Marchelina
, Elza Ibrahim Auerkari

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

E-cadherin (CDH1) gene plays a role in cellular interaction to maintain connection between cells. Loss of CDH1 gene function can affect the development of cancer. Genetic polymorphism CDH1 (-160C> A) is associated with head and neck cancer (HNC). The primary goal of this study is to detect genetic polymorphisms of CDH1 (-160C> A) in HNC patients and Indonesian healthy individual population. The number of samples used is 100 samples consisting of stored DNA samples of 50 healthy individuals and 50 HNC patients. CDHI1 polymorphism was analyzed by the PCR-RFLP method using the HincII restriction enzyme and visualized with electrophoresis. As the result, genetic polymorphisms of CDH1 (-160C> A) were detected in HNC patients by 78% and in healthy individuals by 68%. Neck and head cancer samples has a CC (non-polymorphic) percentage of genotype of 22%, CA ((heterozygous) genotype of 52%, and AA ((homozygous) genotype of 26%. Healthy individual samples has a CC percentage of genotype of 32%, CA genotype of 56%, and AA genotype of 12%. This study shows that genetic polymorphism of CDH1 (-160C> A) increases the risk of HNCs in the Indonesian population.

Original language	English
Title of host publication	3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publication	Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018
Editors	Praswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735418226
DOIs	https://doi.org/10.1063/1.5096755
Publication status	Published - 9 Apr 2019
Event	3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia Duration: 6 Aug 2018 → 8 Aug 2018

Publication series

Name	AIP Conference Proceedings
Volume	2092
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Country/Territory	Indonesia
City	Jakarta
Period	6/08/18 → 8/08/18

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1063/1.5096755

Cite this

Puspitasari, N. A., Midoen, Y. H., Soedarsono, N., Djamal, N., Marchelina, T., & Auerkari, E. I. (2019). Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population. In P. P. D. K. Wulan, M. Gozan, S. Astutiningsih, G. Ramahdita, R. Dhelika, & P. Kreshanti (Eds.), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018 Article 040022 (AIP Conference Proceedings; Vol. 2092). American Institute of Physics Inc.. https://doi.org/10.1063/1.5096755

Puspitasari, Nisa Ajeng ; Midoen, Yurnadi Hanafi ; Soedarsono, Nurtami et al. / Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population. 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. editor / Praswasti P.D.K. Wulan ; Misri Gozan ; Sotya Astutiningsih ; Ghiska Ramahdita ; Radon Dhelika ; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).

@inproceedings{9cf71355045b4b878157c077dbbbdf81,

title = "Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population",

abstract = "E-cadherin (CDH1) gene plays a role in cellular interaction to maintain connection between cells. Loss of CDH1 gene function can affect the development of cancer. Genetic polymorphism CDH1 (-160C> A) is associated with head and neck cancer (HNC). The primary goal of this study is to detect genetic polymorphisms of CDH1 (-160C> A) in HNC patients and Indonesian healthy individual population. The number of samples used is 100 samples consisting of stored DNA samples of 50 healthy individuals and 50 HNC patients. CDHI1 polymorphism was analyzed by the PCR-RFLP method using the HincII restriction enzyme and visualized with electrophoresis. As the result, genetic polymorphisms of CDH1 (-160C> A) were detected in HNC patients by 78\% and in healthy individuals by 68\%. Neck and head cancer samples has a CC (non-polymorphic) percentage of genotype of 22\%, CA ((heterozygous) genotype of 52\%, and AA ((homozygous) genotype of 26\%. Healthy individual samples has a CC percentage of genotype of 32\%, CA genotype of 56\%, and AA genotype of 12\%. This study shows that genetic polymorphism of CDH1 (-160C> A) increases the risk of HNCs in the Indonesian population.",

author = "Puspitasari, \{Nisa Ajeng\} and Midoen, \{Yurnadi Hanafi\} and Nurtami Soedarsono and Niniarty Djamal and Triana Marchelina and Auerkari, \{Elza Ibrahim\}",

note = "Publisher Copyright: {\textcopyright} 2019 Author(s).; 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 ; Conference date: 06-08-2018 Through 08-08-2018",

year = "2019",

month = apr,

day = "9",

doi = "10.1063/1.5096755",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Wulan, \{Praswasti P.D.K.\} and Misri Gozan and Sotya Astutiningsih and Ghiska Ramahdita and Radon Dhelika and Prasetyanugraheni Kreshanti",

booktitle = "3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices",

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}

Puspitasari, NA, Midoen, YH , Soedarsono, N, Djamal, N, Marchelina, T & Auerkari, EI 2019, Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population. in PPDK Wulan, M Gozan, S Astutiningsih, G Ramahdita, R Dhelika & P Kreshanti (eds), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018., 040022, AIP Conference Proceedings, vol. 2092, American Institute of Physics Inc., 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018, Jakarta, Indonesia, 6/08/18. https://doi.org/10.1063/1.5096755

Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population. / Puspitasari, Nisa Ajeng; Midoen, Yurnadi Hanafi ; Soedarsono, Nurtami et al.
3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. ed. / Praswasti P.D.K. Wulan; Misri Gozan; Sotya Astutiningsih; Ghiska Ramahdita; Radon Dhelika; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. 040022 (AIP Conference Proceedings; Vol. 2092).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population

AU - Puspitasari, Nisa Ajeng

AU - Midoen, Yurnadi Hanafi

AU - Soedarsono, Nurtami

AU - Djamal, Niniarty

AU - Marchelina, Triana

AU - Auerkari, Elza Ibrahim

PY - 2019/4/9

Y1 - 2019/4/9

N2 - E-cadherin (CDH1) gene plays a role in cellular interaction to maintain connection between cells. Loss of CDH1 gene function can affect the development of cancer. Genetic polymorphism CDH1 (-160C> A) is associated with head and neck cancer (HNC). The primary goal of this study is to detect genetic polymorphisms of CDH1 (-160C> A) in HNC patients and Indonesian healthy individual population. The number of samples used is 100 samples consisting of stored DNA samples of 50 healthy individuals and 50 HNC patients. CDHI1 polymorphism was analyzed by the PCR-RFLP method using the HincII restriction enzyme and visualized with electrophoresis. As the result, genetic polymorphisms of CDH1 (-160C> A) were detected in HNC patients by 78% and in healthy individuals by 68%. Neck and head cancer samples has a CC (non-polymorphic) percentage of genotype of 22%, CA ((heterozygous) genotype of 52%, and AA ((homozygous) genotype of 26%. Healthy individual samples has a CC percentage of genotype of 32%, CA genotype of 56%, and AA genotype of 12%. This study shows that genetic polymorphism of CDH1 (-160C> A) increases the risk of HNCs in the Indonesian population.

AB - E-cadherin (CDH1) gene plays a role in cellular interaction to maintain connection between cells. Loss of CDH1 gene function can affect the development of cancer. Genetic polymorphism CDH1 (-160C> A) is associated with head and neck cancer (HNC). The primary goal of this study is to detect genetic polymorphisms of CDH1 (-160C> A) in HNC patients and Indonesian healthy individual population. The number of samples used is 100 samples consisting of stored DNA samples of 50 healthy individuals and 50 HNC patients. CDHI1 polymorphism was analyzed by the PCR-RFLP method using the HincII restriction enzyme and visualized with electrophoresis. As the result, genetic polymorphisms of CDH1 (-160C> A) were detected in HNC patients by 78% and in healthy individuals by 68%. Neck and head cancer samples has a CC (non-polymorphic) percentage of genotype of 22%, CA ((heterozygous) genotype of 52%, and AA ((homozygous) genotype of 26%. Healthy individual samples has a CC percentage of genotype of 32%, CA genotype of 56%, and AA genotype of 12%. This study shows that genetic polymorphism of CDH1 (-160C> A) increases the risk of HNCs in the Indonesian population.

UR - https://www.scopus.com/pages/publications/85064816758

U2 - 10.1063/1.5096755

DO - 10.1063/1.5096755

M3 - Conference contribution

AN - SCOPUS:85064816758

T3 - AIP Conference Proceedings

BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices

A2 - Wulan, Praswasti P.D.K.

A2 - Gozan, Misri

A2 - Astutiningsih, Sotya

A2 - Ramahdita, Ghiska

A2 - Dhelika, Radon

A2 - Kreshanti, Prasetyanugraheni

PB - American Institute of Physics Inc.

T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018

Y2 - 6 August 2018 through 8 August 2018

ER -

Puspitasari NA, Midoen YH , Soedarsono N, Djamal N, Marchelina T, Auerkari EI. Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population. In Wulan PPDK, Gozan M, Astutiningsih S, Ramahdita G, Dhelika R, Kreshanti P, editors, 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. American Institute of Physics Inc. 2019. 040022. (AIP Conference Proceedings). doi: 10.1063/1.5096755

Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population

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