TY - GEN
T1 - Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population
AU - Puspitasari, Nisa Ajeng
AU - Midoen, Yurnadi Hanafi
AU - Soedarsono, Nurtami
AU - Djamal, Niniarty
AU - Marchelina, Triana
AU - Auerkari, Elza Ibrahim
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/4/9
Y1 - 2019/4/9
N2 - E-cadherin (CDH1) gene plays a role in cellular interaction to maintain connection between cells. Loss of CDH1 gene function can affect the development of cancer. Genetic polymorphism CDH1 (-160C> A) is associated with head and neck cancer (HNC). The primary goal of this study is to detect genetic polymorphisms of CDH1 (-160C> A) in HNC patients and Indonesian healthy individual population. The number of samples used is 100 samples consisting of stored DNA samples of 50 healthy individuals and 50 HNC patients. CDHI1 polymorphism was analyzed by the PCR-RFLP method using the HincII restriction enzyme and visualized with electrophoresis. As the result, genetic polymorphisms of CDH1 (-160C> A) were detected in HNC patients by 78% and in healthy individuals by 68%. Neck and head cancer samples has a CC (non-polymorphic) percentage of genotype of 22%, CA ((heterozygous) genotype of 52%, and AA ((homozygous) genotype of 26%. Healthy individual samples has a CC percentage of genotype of 32%, CA genotype of 56%, and AA genotype of 12%. This study shows that genetic polymorphism of CDH1 (-160C> A) increases the risk of HNCs in the Indonesian population.
AB - E-cadherin (CDH1) gene plays a role in cellular interaction to maintain connection between cells. Loss of CDH1 gene function can affect the development of cancer. Genetic polymorphism CDH1 (-160C> A) is associated with head and neck cancer (HNC). The primary goal of this study is to detect genetic polymorphisms of CDH1 (-160C> A) in HNC patients and Indonesian healthy individual population. The number of samples used is 100 samples consisting of stored DNA samples of 50 healthy individuals and 50 HNC patients. CDHI1 polymorphism was analyzed by the PCR-RFLP method using the HincII restriction enzyme and visualized with electrophoresis. As the result, genetic polymorphisms of CDH1 (-160C> A) were detected in HNC patients by 78% and in healthy individuals by 68%. Neck and head cancer samples has a CC (non-polymorphic) percentage of genotype of 22%, CA ((heterozygous) genotype of 52%, and AA ((homozygous) genotype of 26%. Healthy individual samples has a CC percentage of genotype of 32%, CA genotype of 56%, and AA genotype of 12%. This study shows that genetic polymorphism of CDH1 (-160C> A) increases the risk of HNCs in the Indonesian population.
UR - http://www.scopus.com/inward/record.url?scp=85064816758&partnerID=8YFLogxK
U2 - 10.1063/1.5096755
DO - 10.1063/1.5096755
M3 - Conference contribution
AN - SCOPUS:85064816758
T3 - AIP Conference Proceedings
BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Wulan, Praswasti P.D.K.
A2 - Gozan, Misri
A2 - Astutiningsih, Sotya
A2 - Ramahdita, Ghiska
A2 - Dhelika, Radon
A2 - Kreshanti, Prasetyanugraheni
PB - American Institute of Physics Inc.
T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Y2 - 6 August 2018 through 8 August 2018
ER -
