E-cadherin (CDH1) gene plays a role in cellular interaction to maintain connection between cells. Loss of CDH1 gene function can affect the development of cancer. Genetic polymorphism CDH1 (-160C> A) is associated with head and neck cancer (HNC). The primary goal of this study is to detect genetic polymorphisms of CDH1 (-160C> A) in HNC patients and Indonesian healthy individual population. The number of samples used is 100 samples consisting of stored DNA samples of 50 healthy individuals and 50 HNC patients. CDHI1 polymorphism was analyzed by the PCR-RFLP method using the HincII restriction enzyme and visualized with electrophoresis. As the result, genetic polymorphisms of CDH1 (-160C> A) were detected in HNC patients by 78% and in healthy individuals by 68%. Neck and head cancer samples has a CC (non-polymorphic) percentage of genotype of 22%, CA ((heterozygous) genotype of 52%, and AA ((homozygous) genotype of 26%. Healthy individual samples has a CC percentage of genotype of 32%, CA genotype of 56%, and AA genotype of 12%. This study shows that genetic polymorphism of CDH1 (-160C> A) increases the risk of HNCs in the Indonesian population.