Polymorphism detection of gene CDH1(-160C > A) in head and neck cancer patient and Indonesian healthy individual population

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Abstract

E-cadherin (CDH1) gene plays a role in cellular interaction to maintain connection between cells. Loss of CDH1 gene function can affect the development of cancer. Genetic polymorphism CDH1 (-160C> A) is associated with head and neck cancer (HNC). The primary goal of this study is to detect genetic polymorphisms of CDH1 (-160C> A) in HNC patients and Indonesian healthy individual population. The number of samples used is 100 samples consisting of stored DNA samples of 50 healthy individuals and 50 HNC patients. CDHI1 polymorphism was analyzed by the PCR-RFLP method using the HincII restriction enzyme and visualized with electrophoresis. As the result, genetic polymorphisms of CDH1 (-160C> A) were detected in HNC patients by 78% and in healthy individuals by 68%. Neck and head cancer samples has a CC (non-polymorphic) percentage of genotype of 22%, CA ((heterozygous) genotype of 52%, and AA ((homozygous) genotype of 26%. Healthy individual samples has a CC percentage of genotype of 32%, CA genotype of 56%, and AA genotype of 12%. This study shows that genetic polymorphism of CDH1 (-160C> A) increases the risk of HNCs in the Indonesian population.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Country/TerritoryIndonesia
CityJakarta
Period6/08/188/08/18

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