Polyclonal antibodies production from porcine gelatin and its preliminary study for immunosensor applications

A. I. Astuti, R. D. Soejoedono, E. Saepudin, L. D. Assaat, T. A. Ivandini

Research output: Contribution to journalConference articlepeer-review

Abstract

Gelatin was extracted from porcine skin and used as an antigen to produce the polyclonal antibodies of porcine gelatin using New Zealand white rabbits for application in the development of an ELISA-based immunosensor for porcine gelatin recognition. The extraction of porcine gelatin from porcine skin was successfully performed with 20.52 % of yield. The characterization performed by using UV and IR spectroscopy indicated that the purified gelatin has a comparable quality against the commercial standard of porcine gelatin. Further analysis of the gelatin with SDS-PAGE method observed five separation lines with molecular weight bands of 57.06; 49.50; 42.94; 33.89; and 29.40 KDa, indicating that the gelatin can be used as an antigen to produce the polyclonal antibodies of porcine gelatin. After five injections of gelatin into the rabbit, ELISA characterization of the purified serum of the blood rabbit showed that the antibody with a concentration of 0.289 mg/mL could be produced.

Original languageEnglish
Article number012007
JournalIOP Conference Series: Materials Science and Engineering
Volume763
Issue number1
DOIs
Publication statusPublished - 28 Apr 2020
Event3rd International Symposium on Current Progress in Functional Materials 2018, ISCPFM 2018 - Depok, Indonesia
Duration: 8 Aug 20189 Aug 2018

Keywords

  • ELISA
  • immunosensor
  • polyclonal antibody
  • porcine gelatin
  • rabbit

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