Polyclonal antibodies production from porcine gelatin and its preliminary study for immunosensor applications

A. I. Astuti; R. D. Soejoedono; E. Saepudin; L. D. Assaat; T. A. Ivandini

doi:10.1088/1757-899X/763/1/012007

Polyclonal antibodies production from porcine gelatin and its preliminary study for immunosensor applications

A. I. Astuti, R. D. Soejoedono, E. Saepudin, L. D. Assaat, T. A. Ivandini

Department of Chemistry

Research output: Contribution to journal › Conference article › peer-review

Abstract

Gelatin was extracted from porcine skin and used as an antigen to produce the polyclonal antibodies of porcine gelatin using New Zealand white rabbits for application in the development of an ELISA-based immunosensor for porcine gelatin recognition. The extraction of porcine gelatin from porcine skin was successfully performed with 20.52 % of yield. The characterization performed by using UV and IR spectroscopy indicated that the purified gelatin has a comparable quality against the commercial standard of porcine gelatin. Further analysis of the gelatin with SDS-PAGE method observed five separation lines with molecular weight bands of 57.06; 49.50; 42.94; 33.89; and 29.40 KDa, indicating that the gelatin can be used as an antigen to produce the polyclonal antibodies of porcine gelatin. After five injections of gelatin into the rabbit, ELISA characterization of the purified serum of the blood rabbit showed that the antibody with a concentration of 0.289 mg/mL could be produced.

Original language	English
Article number	012007
Journal	IOP Conference Series: Materials Science and Engineering
Volume	763
Issue number	1
DOIs	https://doi.org/10.1088/1757-899X/763/1/012007
Publication status	Published - 28 Apr 2020
Event	3rd International Symposium on Current Progress in Functional Materials 2018, ISCPFM 2018 - Depok, Indonesia Duration: 8 Aug 2018 → 9 Aug 2018

Keywords

ELISA
immunosensor
polyclonal antibody
porcine gelatin
rabbit

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10.1088/1757-899X/763/1/012007

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title = "Polyclonal antibodies production from porcine gelatin and its preliminary study for immunosensor applications",

abstract = "Gelatin was extracted from porcine skin and used as an antigen to produce the polyclonal antibodies of porcine gelatin using New Zealand white rabbits for application in the development of an ELISA-based immunosensor for porcine gelatin recognition. The extraction of porcine gelatin from porcine skin was successfully performed with 20.52 % of yield. The characterization performed by using UV and IR spectroscopy indicated that the purified gelatin has a comparable quality against the commercial standard of porcine gelatin. Further analysis of the gelatin with SDS-PAGE method observed five separation lines with molecular weight bands of 57.06; 49.50; 42.94; 33.89; and 29.40 KDa, indicating that the gelatin can be used as an antigen to produce the polyclonal antibodies of porcine gelatin. After five injections of gelatin into the rabbit, ELISA characterization of the purified serum of the blood rabbit showed that the antibody with a concentration of 0.289 mg/mL could be produced.",

keywords = "ELISA, immunosensor, polyclonal antibody, porcine gelatin, rabbit",

author = "Astuti, {A. I.} and Soejoedono, {R. D.} and E. Saepudin and Assaat, {L. D.} and Ivandini, {T. A.}",

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TY - JOUR

T1 - Polyclonal antibodies production from porcine gelatin and its preliminary study for immunosensor applications

AU - Astuti, A. I.

AU - Soejoedono, R. D.

AU - Saepudin, E.

AU - Assaat, L. D.

AU - Ivandini, T. A.

PY - 2020/4/28

Y1 - 2020/4/28

N2 - Gelatin was extracted from porcine skin and used as an antigen to produce the polyclonal antibodies of porcine gelatin using New Zealand white rabbits for application in the development of an ELISA-based immunosensor for porcine gelatin recognition. The extraction of porcine gelatin from porcine skin was successfully performed with 20.52 % of yield. The characterization performed by using UV and IR spectroscopy indicated that the purified gelatin has a comparable quality against the commercial standard of porcine gelatin. Further analysis of the gelatin with SDS-PAGE method observed five separation lines with molecular weight bands of 57.06; 49.50; 42.94; 33.89; and 29.40 KDa, indicating that the gelatin can be used as an antigen to produce the polyclonal antibodies of porcine gelatin. After five injections of gelatin into the rabbit, ELISA characterization of the purified serum of the blood rabbit showed that the antibody with a concentration of 0.289 mg/mL could be produced.

AB - Gelatin was extracted from porcine skin and used as an antigen to produce the polyclonal antibodies of porcine gelatin using New Zealand white rabbits for application in the development of an ELISA-based immunosensor for porcine gelatin recognition. The extraction of porcine gelatin from porcine skin was successfully performed with 20.52 % of yield. The characterization performed by using UV and IR spectroscopy indicated that the purified gelatin has a comparable quality against the commercial standard of porcine gelatin. Further analysis of the gelatin with SDS-PAGE method observed five separation lines with molecular weight bands of 57.06; 49.50; 42.94; 33.89; and 29.40 KDa, indicating that the gelatin can be used as an antigen to produce the polyclonal antibodies of porcine gelatin. After five injections of gelatin into the rabbit, ELISA characterization of the purified serum of the blood rabbit showed that the antibody with a concentration of 0.289 mg/mL could be produced.

KW - ELISA

KW - immunosensor

KW - polyclonal antibody

KW - porcine gelatin

KW - rabbit

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