@article{6b58353dcd454f4c9712746eaa862b28,
title = "Polyclonal antibodies production from porcine gelatin and its preliminary study for immunosensor applications",
abstract = "Gelatin was extracted from porcine skin and used as an antigen to produce the polyclonal antibodies of porcine gelatin using New Zealand white rabbits for application in the development of an ELISA-based immunosensor for porcine gelatin recognition. The extraction of porcine gelatin from porcine skin was successfully performed with 20.52 % of yield. The characterization performed by using UV and IR spectroscopy indicated that the purified gelatin has a comparable quality against the commercial standard of porcine gelatin. Further analysis of the gelatin with SDS-PAGE method observed five separation lines with molecular weight bands of 57.06; 49.50; 42.94; 33.89; and 29.40 KDa, indicating that the gelatin can be used as an antigen to produce the polyclonal antibodies of porcine gelatin. After five injections of gelatin into the rabbit, ELISA characterization of the purified serum of the blood rabbit showed that the antibody with a concentration of 0.289 mg/mL could be produced.",
keywords = "ELISA, immunosensor, polyclonal antibody, porcine gelatin, rabbit",
author = "Astuti, {A. I.} and Soejoedono, {R. D.} and E. Saepudin and Assaat, {L. D.} and Ivandini, {T. A.}",
note = "Funding Information: Ac k n o wle d g e m e n ts This work was funded by Hibah PITTA Universitas Indonesia with contract number of 2274/UN2.R3.1/HKP.05.00/2018 Re fe re n c e s [1] YiJB,KimY T,BaeHJ,WhitesideWSandParkHJ2006J.FoodSci.71E376–83 [2] GudmundssonM2002J.FoodSci.672172–6 [3] KarimAAandBhatR2008TrendsFoodSci.Technol.19644–56 [4] Venugopal V 2008 Marine Products for Healthcare: Functional and Bioactive Nutraceutical CompoundsfromtheOcean(BocaRaton: CRCrPess) [5] DoiH,WatanabeE,ShibataH andTanabeS2009J.Agric.FoodChem.571721–6 [6] CaiH,GuX,ScanlanMS,RamatlapengDHandLivelyC R 2012J.FoodCompos.Anal.25 83–7 [7] HidakaSandLiuSY2003J.FoodCompos.Anal.16477–83 [8] RaraswatiMA,TriyanaKandRohmanA2014J.FoodPharm.Sci.21–6 [9] NurAziraT, Amin Iand CheMan Y B 2012 InternationalFood Research Journal(IFRJ)19 1175–80 [10] Venien A and Levieux D 2005 J. Pharm. Biomed. Anal. 39 418–24 [11] Bellanti J A 1993 Immunologi III, tranlastor A S Wahab (Yogyakarta: Gadjah Mada University Press) [12] Syamsuri A and Wardani A K 2013 Jurnal Pangan dan Argoindustri 1 36–45 [13] Raja Mohd Hafidz R N, Yaakob C M, Amin I and Nurfaidzan A 2011 International Food Research Journal (IFRJ) 18 813–7 [14] Ross-Murphy S B 1997 The Imaging Sci. J. 45 205–9 Publisher Copyright: {\textcopyright} 2020 Published under licence by IOP Publishing Ltd.; 3rd International Symposium on Current Progress in Functional Materials 2018, ISCPFM 2018 ; Conference date: 08-08-2018 Through 09-08-2018",
year = "2020",
month = apr,
day = "28",
doi = "10.1088/1757-899X/763/1/012007",
language = "English",
volume = "763",
journal = "IOP Conference Series: Materials Science and Engineering",
issn = "1757-8981",
publisher = "IOP Publishing Ltd.",
number = "1",
}