Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta

Seruni K.U. Freisleben, Jenny Hidayat, Hans Joachim Freisleben, Soehardjo Poertadji, Budi Kurniawan, Na Peng Bo, Sri Handayani, Pustika Amalia Wahidiyat, Rondang R. Soegianto

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Background: Over the last 10 years, we have investigated thalassemia patients in Jakarta to obtain a comprehensive picture of iron overload, oxidative stress, and cell damage. Methods: In blood samples from 15 transfusion-dependent patients (group T), 5 non-transfused patients (group N) and 10 controls (group C), plasma lipids and lipoproteins, lipid-soluble vitamin E, malondialdehyde (MDA) and thiol status were measured. Isolated eryhtrocyte membranes were investigated with electron paramagnetic resonance (EPR) spectroscopy using doxyl-stearic acid and maleimido-proxyl spin lables. Data were analyzed statistically with ANOVA. Results: Plasma triglycerides were higher and cholesterol levels were lower in thalassemic patients compared to controls. Vitamin E, group C: 21.8 vs T: 6.2 μmol/L) and reactive thiols (C: 144 vs. T: 61 μmol/L) were considerably lower in transfused patients, who exert clear signs of oxidative stress (MDA, C: 1.96 vs T: 9.2 μmol/L) and of tissue cell damage, i.e., high transaminases plasma levels. Non-transfused thalassemia patients have slight signs of oxidative stress, but no significant indication of cell damage. Erythrocyte membrane parameters from EPR spectroscopy differ considerably between all groups. In transfusion-dependent patients the structure of the erythrocyte membrane and the gradients of polarity and fluidity are destroyed in lipid domains; binding capacity of protein thiols in the membrane is lower and immobilized. Conclusion: In tranfusion-dependent thalassemic patients, plasma lipid pattern and oxidative stress are associated with structural damage of isolated erythrocyte membranes as measured by EPR spectroscopy with lipid and proteinthiol spin labels.

Original languageEnglish
Pages (from-to)178-184
Number of pages7
JournalMedical Journal of Indonesia
Volume20
Issue number3
DOIs
Publication statusPublished - 1 Aug 2011

Keywords

  • Electron paramagnetic resonance spectroscopy
  • Erythrocyte membrane
  • Lipoproteins
  • Oxidative stress
  • Plasma lipids
  • Thalassemia

Fingerprint Dive into the research topics of 'Plasma lipid pattern and red cell membrane structure in β-thalassemia patients in Jakarta'. Together they form a unique fingerprint.

Cite this