Plasma 3-nitrotyrosine, urinary 8-isoprostane and 8-OHdG among healthy Japanese people

Noriko Sakano, Noriko Takahashi, Da Hong Wang, Rani Sauriasari, Kei Takemoto, Sakiko Kanbara, Yoshie Sato, Tomoko Takigawa, Jiro Takaki, Keiki Ogino

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

To examine the relation between lifestyle factors and oxidative stress biomarkers, this study analysed plasma 3-nitrotyrosine (3-NT), urinary 8-isoprostane and 8-hydroxy-2′-deoxyguanosine (8-OHdG) of 323 healthy Japanese without any disease. Plasma 3-NT was significantly increased by excessive exercise (p=0.010), but it was not significantly different in terms of sex, age (< 40, ≥40), BMI (<18.5, 18.5-24.9, ≥25.0), smoking (non-smokers, smokers) and alcohol drinking per week (non-drinkers, <10 units, ≥10 units). Urinary 8-isoprostane was significantly associated with alcohol drinking (p<0.01) and sex (p<0.01), although it had no significant relevance to age and exercise. Moreover, urinary 8-OHdG was positively associated with age (p<0.05) and negatively associated with BMI (p<0.05) and fasting insulin (p<0.001). However, it was not related with sex, smoking, alcohol drinking and exercise. In conclusion, the present results suggest that 3-NT, 8-isoprostane and 8-OHdG seem to be useful biomarkers for early prediction of lifestyle-related disease risk at the population level.

Original languageEnglish
Pages (from-to)183-192
Number of pages10
JournalFree Radical Research
Volume43
Issue number2
DOIs
Publication statusPublished - 2009

Keywords

  • 3-Nitrotyrosine
  • 8-OHdG
  • 8-isoprostane
  • Biomarkers
  • Lipid peroxidation
  • Reactive oxygen species

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