TY - JOUR
T1 - Placenta-derived, cellular messenger RNA expression in the maternal blood of preeclamptic women
AU - Okazaki, Shiho
AU - Sekizawa, Akihiko
AU - Purwosunu, Yuditiya
AU - Farina, Antonio
AU - Wibowo, Noroyono
AU - Okai, Takashi
PY - 2007/11
Y1 - 2007/11
N2 - OBJECTIVE: To perform gene expression profiling and real-time quantitative reverse-transcription polymerase chain reaction (PCR) analysis to identify biomarkers of preeclampsia in cellular messenger RNA (mRNA) from maternal blood. METHODS: We performed a microarray analysis with five maternal blood samples from women with preeclampsia and five matched control subjects. Up-regulated gene expression was further analyzed through reverse-transcription PCR analysis with 28 consecutive blood samples from women affected with preeclampsia and 29 controls. RESULTS: Both pregnancy-specific β1 glycoprotein and trophoblast glycoprotein were selected based on microarray analysis. Reverse-transcription PCR analysis detected significantly increased mRNA concentrations among women in the preeclampsia group. When stratified according to mild or severe preeclampsia, 19.2-fold and 41.8-fold increases in pregnancy-specific β1 glycoprotein and 8.3-fold and 10.6-fold increases in trophoblast glycoprotein were observed, respectively. Among women with hemolysis, elevated liver enzymes, and low platelet count syndrome, 51.6-fold and 13.1-fold increases in pregnancy-specific β1 glycoprotein and trophoblast glycoprotein were observed, respectively. In the preeclampsia group, pregnancy-specific β1 glycoprotein correlated with severity of proteinuria (P<.001) and systolic blood pressure (P=.01). CONCLUSION: The mRNA expression of pregnancy-specific β1 glycoprotein and trophoblast glycoprotein is up-regulated in cells circulating within blood from women with preeclampsia, and pregnancy-specific β1 glycoprotein expression is positively correlated with the clinical severity of preeclampsia.
AB - OBJECTIVE: To perform gene expression profiling and real-time quantitative reverse-transcription polymerase chain reaction (PCR) analysis to identify biomarkers of preeclampsia in cellular messenger RNA (mRNA) from maternal blood. METHODS: We performed a microarray analysis with five maternal blood samples from women with preeclampsia and five matched control subjects. Up-regulated gene expression was further analyzed through reverse-transcription PCR analysis with 28 consecutive blood samples from women affected with preeclampsia and 29 controls. RESULTS: Both pregnancy-specific β1 glycoprotein and trophoblast glycoprotein were selected based on microarray analysis. Reverse-transcription PCR analysis detected significantly increased mRNA concentrations among women in the preeclampsia group. When stratified according to mild or severe preeclampsia, 19.2-fold and 41.8-fold increases in pregnancy-specific β1 glycoprotein and 8.3-fold and 10.6-fold increases in trophoblast glycoprotein were observed, respectively. Among women with hemolysis, elevated liver enzymes, and low platelet count syndrome, 51.6-fold and 13.1-fold increases in pregnancy-specific β1 glycoprotein and trophoblast glycoprotein were observed, respectively. In the preeclampsia group, pregnancy-specific β1 glycoprotein correlated with severity of proteinuria (P<.001) and systolic blood pressure (P=.01). CONCLUSION: The mRNA expression of pregnancy-specific β1 glycoprotein and trophoblast glycoprotein is up-regulated in cells circulating within blood from women with preeclampsia, and pregnancy-specific β1 glycoprotein expression is positively correlated with the clinical severity of preeclampsia.
UR - http://www.scopus.com/inward/record.url?scp=35848954710&partnerID=8YFLogxK
U2 - 10.1097/01.AOG.0000286761.11436.67
DO - 10.1097/01.AOG.0000286761.11436.67
M3 - Article
C2 - 17978129
AN - SCOPUS:35848954710
SN - 0029-7844
VL - 110
SP - 1130
EP - 1136
JO - Obstetrics and Gynecology
JF - Obstetrics and Gynecology
IS - 5
ER -