Piperine in the prevention of the decreased tamoxifen sensitivity in MCF-7 breast cancer cell line

Sandy Vitria Kurniawan, Lies Sugiarti, Septelia Inawati Wanandi, Melva Louisa

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Objective: This study was designed to analyze the role of piperine in modulating P-glycoprotein mRNA expression when added in combination with tamoxifen to breast cancer cells in culture. Methods: MCF-7 breast cancer cells were treated with 1 µM tamoxifen with or without piperine (12.5, 25, or 50 µM) or verapamil 50 µM (P-glycoprotein inhibitor positive control) for up to 12 days. We assessed the cell viability and isolated total RNA from them. We quantified P-glycoprotein expressions using quantitative reverse transcription polymerase chain reaction. Results: Administration of various doses of piperine decreased MCF-7 breast cancer cell viability. Piperine, when given in combination with tamoxifen, decreased the expression of P-glycoprotein mRNA in cells compared with the expression in cells treated with tamoxifen only. The effects were shown to be dose dependent. Conclusion: Piperine prevents the development of breast cancer cell tamoxifen resistance, probably through its inhibition of P-glycoprotein expression.

Original languageEnglish
Pages (from-to)335-337
Number of pages3
JournalInternational Journal of Applied Pharmaceutics
Issue numberSpecial Issue 1
Publication statusPublished - 1 Dec 2018


  • P-glycoprotein
  • Piperine
  • Tamoxifen


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