Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells

Elvira Lesmana; Ade Arsianti; Trivani Putri; Priscilla Aya Maheswari Subroto

doi:10.1063/1.5096721

Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells

Elvira Lesmana, Ade Arsianti, Trivani Putri, Priscilla Aya Maheswari Subroto

Department of Medical Chemistry Pre Clinic

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In this research, we develop Indonesia marine macroalgae of Eucheuma sp. as an anti-breast cancer agent. Macroalgae Eucheuma sp. which was collected from Labuhan Aji beach, Lombok, Nusa Tenggara Barat, Indonesia, were extracted into four different organic solvents, which are n-hexane, ethyl acetate, chloroform and ethanol. The extracts were subjected to Phytochemistry test and were analyzed by Thin Layer Chromatography (TLC). Subsequently, cytotoxic activity of n-hexane, chloroform, ethyl acetate, and ethanol extracts of Eucheuma sp. were evaluated as a growth inhibitor for breast MCF-7 cancer cells. Based on the phytochemical analysis, the extracts of Eucheuma sp. containing secondary metabolite of flavonoid, triterpenoid, alkaloid and tannin, while TLC analysis indicated that the extracts were composed of one to four chemical compounds. Extracts of Eucheuma sp. demonstrated a strong cytotoxic activity against breast MCF-7 cancer cells. The results suggesting that macroalgae Eucheuma sp. should be developed as a promising anti-breast cancer agent.

Original language	English
Title of host publication	3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publication	Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018
Editors	Praswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735418226
DOIs	https://doi.org/10.1063/1.5096721
Publication status	Published - 9 Apr 2019
Event	3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia Duration: 6 Aug 2018 → 8 Aug 2018

Publication series

Name	AIP Conference Proceedings
Volume	2092
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Country/Territory	Indonesia
City	Jakarta
Period	6/08/18 → 8/08/18

Keywords

breast MCF-7 cells
cytotoxic activity
Eucheuma sp.
macroalgae
MTT assay
phytochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1063/1.5096721

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Lesmana, E., Arsianti, A., Putri, T., & Subroto, P. A. M. (2019). Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells. In P. P. D. K. Wulan, M. Gozan, S. Astutiningsih, G. Ramahdita, R. Dhelika, & P. Kreshanti (Eds.), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018 Article 030017 (AIP Conference Proceedings; Vol. 2092). American Institute of Physics Inc.. https://doi.org/10.1063/1.5096721

Lesmana, Elvira ; Arsianti, Ade ; Putri, Trivani et al. / Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells. 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. editor / Praswasti P.D.K. Wulan ; Misri Gozan ; Sotya Astutiningsih ; Ghiska Ramahdita ; Radon Dhelika ; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).

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title = "Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells",

abstract = "In this research, we develop Indonesia marine macroalgae of Eucheuma sp. as an anti-breast cancer agent. Macroalgae Eucheuma sp. which was collected from Labuhan Aji beach, Lombok, Nusa Tenggara Barat, Indonesia, were extracted into four different organic solvents, which are n-hexane, ethyl acetate, chloroform and ethanol. The extracts were subjected to Phytochemistry test and were analyzed by Thin Layer Chromatography (TLC). Subsequently, cytotoxic activity of n-hexane, chloroform, ethyl acetate, and ethanol extracts of Eucheuma sp. were evaluated as a growth inhibitor for breast MCF-7 cancer cells. Based on the phytochemical analysis, the extracts of Eucheuma sp. containing secondary metabolite of flavonoid, triterpenoid, alkaloid and tannin, while TLC analysis indicated that the extracts were composed of one to four chemical compounds. Extracts of Eucheuma sp. demonstrated a strong cytotoxic activity against breast MCF-7 cancer cells. The results suggesting that macroalgae Eucheuma sp. should be developed as a promising anti-breast cancer agent.",

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Lesmana, E, Arsianti, A, Putri, T & Subroto, PAM 2019, Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells. in PPDK Wulan, M Gozan, S Astutiningsih, G Ramahdita, R Dhelika & P Kreshanti (eds), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018., 030017, AIP Conference Proceedings, vol. 2092, American Institute of Physics Inc., 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018, Jakarta, Indonesia, 6/08/18. https://doi.org/10.1063/1.5096721

Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells. / Lesmana, Elvira; Arsianti, Ade; Putri, Trivani et al.
3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. ed. / Praswasti P.D.K. Wulan; Misri Gozan; Sotya Astutiningsih; Ghiska Ramahdita; Radon Dhelika; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. 030017 (AIP Conference Proceedings; Vol. 2092).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells

AU - Lesmana, Elvira

AU - Arsianti, Ade

AU - Putri, Trivani

AU - Subroto, Priscilla Aya Maheswari

PY - 2019/4/9

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N2 - In this research, we develop Indonesia marine macroalgae of Eucheuma sp. as an anti-breast cancer agent. Macroalgae Eucheuma sp. which was collected from Labuhan Aji beach, Lombok, Nusa Tenggara Barat, Indonesia, were extracted into four different organic solvents, which are n-hexane, ethyl acetate, chloroform and ethanol. The extracts were subjected to Phytochemistry test and were analyzed by Thin Layer Chromatography (TLC). Subsequently, cytotoxic activity of n-hexane, chloroform, ethyl acetate, and ethanol extracts of Eucheuma sp. were evaluated as a growth inhibitor for breast MCF-7 cancer cells. Based on the phytochemical analysis, the extracts of Eucheuma sp. containing secondary metabolite of flavonoid, triterpenoid, alkaloid and tannin, while TLC analysis indicated that the extracts were composed of one to four chemical compounds. Extracts of Eucheuma sp. demonstrated a strong cytotoxic activity against breast MCF-7 cancer cells. The results suggesting that macroalgae Eucheuma sp. should be developed as a promising anti-breast cancer agent.

AB - In this research, we develop Indonesia marine macroalgae of Eucheuma sp. as an anti-breast cancer agent. Macroalgae Eucheuma sp. which was collected from Labuhan Aji beach, Lombok, Nusa Tenggara Barat, Indonesia, were extracted into four different organic solvents, which are n-hexane, ethyl acetate, chloroform and ethanol. The extracts were subjected to Phytochemistry test and were analyzed by Thin Layer Chromatography (TLC). Subsequently, cytotoxic activity of n-hexane, chloroform, ethyl acetate, and ethanol extracts of Eucheuma sp. were evaluated as a growth inhibitor for breast MCF-7 cancer cells. Based on the phytochemical analysis, the extracts of Eucheuma sp. containing secondary metabolite of flavonoid, triterpenoid, alkaloid and tannin, while TLC analysis indicated that the extracts were composed of one to four chemical compounds. Extracts of Eucheuma sp. demonstrated a strong cytotoxic activity against breast MCF-7 cancer cells. The results suggesting that macroalgae Eucheuma sp. should be developed as a promising anti-breast cancer agent.

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KW - macroalgae

KW - MTT assay

KW - phytochemistry

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M3 - Conference contribution

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BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices

A2 - Wulan, Praswasti P.D.K.

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Lesmana E, Arsianti A, Putri T, Subroto PAM. Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells. In Wulan PPDK, Gozan M, Astutiningsih S, Ramahdita G, Dhelika R, Kreshanti P, editors, 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. American Institute of Physics Inc. 2019. 030017. (AIP Conference Proceedings). doi: 10.1063/1.5096721

Phytochemistry test and cytotoxic activity of macroalgae Eucheuma Sp. as a growth inhibitor of breast MCF-7 cancer cells

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Keywords

UN SDGs

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