TY - JOUR
T1 - Phytochemistry and Cytotoxicity Analysis of Kemang (Mangifera kemanga) Fruit Extract on HeLa Cervical Cancer Cell Line
AU - Catrawardhana, Prajnadiyan
AU - Saharso, Ersal Rasyid
AU - Mushlih, Yusuf
AU - Hapsari, Yatri
AU - Fadilah, Fadilah
N1 - Funding Information:
This work was supported by PUTI Grant (NKB-916/UN2.RST/HKP.05.00/2020) from Universitas Indonesia.
Publisher Copyright:
© RJPT All right reserved.
PY - 2022/4
Y1 - 2022/4
N2 - Mango extract has been proven in its anticancer effect against cervical cancer, however kemang (Mangifera kemanga), despite being a relative of mango, has not been thoroughly researched although expected to give the same effect. Objective: To identify the contents contained in the ethanol, ethyl acetate, and n-hexane extract of kemang flesh and examine its cytotoxic effect on HeLa cervical cancer cells. Methods: Kemang flesh was extracted using ethanol, ethyl acetate, and n-hexane. Phytochemical tests and thin-layer chromatography on the extracts were conducted to identify the phytochemical contents. MTT assay was carried out using the extracts against HeLa cells to find out the cytotoxic effect of the samples in IC50 values. Results: Phytochemical tests revealed that kemang flesh extract contains flavonoid, tannin, triterpenoid, and alkaloid. Thin-layer chromatography test with nonpolar eluent showed one spot with Rf of 0.82 in ethanol extract; six spots with Rf of 0.16, 0.36, 0.49, 0.76, 0.82, and 0.94 in ethyl acetate extract; and six spots with Rf of 0.36, 0.48, 0.63, 0.75, 0.83, and 0.93 in n-hexane extract. MTT assay obtained IC50 values for HeLa cells, respectively for ethanol, ethyl acetate, and n-hexane extracts, were 44.34, 16.41, and 43.23ppm. Conclusion: Kemang fruit extract has potential as an anticancer agent against cervical cancer.
AB - Mango extract has been proven in its anticancer effect against cervical cancer, however kemang (Mangifera kemanga), despite being a relative of mango, has not been thoroughly researched although expected to give the same effect. Objective: To identify the contents contained in the ethanol, ethyl acetate, and n-hexane extract of kemang flesh and examine its cytotoxic effect on HeLa cervical cancer cells. Methods: Kemang flesh was extracted using ethanol, ethyl acetate, and n-hexane. Phytochemical tests and thin-layer chromatography on the extracts were conducted to identify the phytochemical contents. MTT assay was carried out using the extracts against HeLa cells to find out the cytotoxic effect of the samples in IC50 values. Results: Phytochemical tests revealed that kemang flesh extract contains flavonoid, tannin, triterpenoid, and alkaloid. Thin-layer chromatography test with nonpolar eluent showed one spot with Rf of 0.82 in ethanol extract; six spots with Rf of 0.16, 0.36, 0.49, 0.76, 0.82, and 0.94 in ethyl acetate extract; and six spots with Rf of 0.36, 0.48, 0.63, 0.75, 0.83, and 0.93 in n-hexane extract. MTT assay obtained IC50 values for HeLa cells, respectively for ethanol, ethyl acetate, and n-hexane extracts, were 44.34, 16.41, and 43.23ppm. Conclusion: Kemang fruit extract has potential as an anticancer agent against cervical cancer.
KW - HeLa
KW - In-vitro Test
KW - Kemang (Mangifera kemanga) Fruit Extract
KW - Phytochemical test
UR - http://www.scopus.com/inward/record.url?scp=85130791421&partnerID=8YFLogxK
U2 - 10.52711/0974-360X.2022.00288
DO - 10.52711/0974-360X.2022.00288
M3 - Article
AN - SCOPUS:85130791421
SN - 0974-3618
VL - 15
SP - 1721
EP - 1726
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 4
ER -