TY - JOUR
T1 - Phytochemicals and antioxidant activities evaluation of origanum vulgare (L.) stem bark extracts
AU - Rosmalena,
AU - Widyastuti, Putu Ayu
AU - Yazid, Fatmawaty
AU - Ambarwati, Neneng Siti Silfi
AU - Ahmad, Islamudin
N1 - Funding Information:
We would like to express our gratitude to International Indexed Publication Grant for Students Final Projects from the University of Indonesia 2018, which have supported this research. This study was supported by the grant of Publikasi Terindeks Internasional untuk Tugas Akhir Mahasiswa (PITTA) 2018, with grant number: 2124/UN2. R3.1/HKP.05.00/2018
Publisher Copyright:
© 2021 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
PY - 2021/7
Y1 - 2021/7
N2 - The present study aimed to evaluate phytochemical and antioxidant activity (in vitro and in vivo) of Origanum vulgare (L.) ethanolic extract. The phytochemical test was assessed using the Clule method in ethanol, ethyl acetate, and hexane. In vitro evaluation of antioxidant activity was determined by radical scavenging assay using DPPH (2,2-diphenyl-1-picrylhydrazyl) as an artificial free radical activity. In vivo test was conducted to evaluate the effect of malondialdehyde (MDA) level in blood plasma during maximum physical activity treatment. In vivo test was done using 25 male Sprague Dawley rats in pre and post-test control group design. The phytochemical test of O. vulgare ethanol extract was showed some compounds, such as a flavonoid, alkaloid, triterpenoid/steroid, essential oil, and tannin, then in ethyl acetate and hexane. In vitro assay showed that O. vulgare extract has strong antioxidant activity with an IC50 value of 133.47 µg/mL. While in the in vivo test, the most effective dosage is 20 mg/200 gr B.W., represented by a significant decrease of MDA level (0.509 nmol/mL) before and after treatment. So, the ethanolic extract of clove has potency as an herbal antioxidant because of the low level of IC50 and can decrease the MDA level.
AB - The present study aimed to evaluate phytochemical and antioxidant activity (in vitro and in vivo) of Origanum vulgare (L.) ethanolic extract. The phytochemical test was assessed using the Clule method in ethanol, ethyl acetate, and hexane. In vitro evaluation of antioxidant activity was determined by radical scavenging assay using DPPH (2,2-diphenyl-1-picrylhydrazyl) as an artificial free radical activity. In vivo test was conducted to evaluate the effect of malondialdehyde (MDA) level in blood plasma during maximum physical activity treatment. In vivo test was done using 25 male Sprague Dawley rats in pre and post-test control group design. The phytochemical test of O. vulgare ethanol extract was showed some compounds, such as a flavonoid, alkaloid, triterpenoid/steroid, essential oil, and tannin, then in ethyl acetate and hexane. In vitro assay showed that O. vulgare extract has strong antioxidant activity with an IC50 value of 133.47 µg/mL. While in the in vivo test, the most effective dosage is 20 mg/200 gr B.W., represented by a significant decrease of MDA level (0.509 nmol/mL) before and after treatment. So, the ethanolic extract of clove has potency as an herbal antioxidant because of the low level of IC50 and can decrease the MDA level.
KW - 2
KW - 2-diphenyl-1-picrylhydrazyl
KW - Antioxidant activity
KW - Malondialdehyde
KW - Origanum vulgare (L.)
KW - Phytochemical
UR - http://www.scopus.com/inward/record.url?scp=85110932652&partnerID=8YFLogxK
U2 - 10.5530/pj.2021.13.124
DO - 10.5530/pj.2021.13.124
M3 - Article
AN - SCOPUS:85110932652
SN - 0975-3575
VL - 13
SP - 965
EP - 970
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 4
ER -