TY - JOUR
T1 - Phytochemical test and cytotoxic activity of macroalgae eucheuma cottonii against cervical HeLa cells
AU - Arsianti, Ade
AU - Aziza, Yully Astika Nugrahayning
AU - Kurniasari, Kartika Dwi
AU - Mandasari, Baiq Kirana Dyahningrum
AU - Masita, Riathul
AU - Zulfa, Futihati Ruhama
AU - Dewi, Micheylla Kusumaning
AU - Zagloel, Cut Raisya Zahira
AU - Azizah, Norma Nur
AU - Putrianingsih, Rista
N1 - Publisher Copyright:
© 2018 Phcog.Net.
PY - 2018
Y1 - 2018
N2 - Intoduction: Marine resource of macroalgae Eucheuma cottonii from East Lombok, Nusa Tenggara Barat, Indonesia, have potential as anti-cervical cancer agent. Objectives: Finding cytotoxic activity of Eucheuma cottonii hexane, ethylacetate, chloroform and ethanol extracts against cervical HeLa cells by MTT cell proliferation assay. Methods: The extracts was tested in phytochemical and cytotoxic activity test. Phytochemical test to identify composition of secondary metabolite such as flavonoid, alkaloid, saponins, tannin, triterpenoid, steroid and glycoside. The amount of substances contained in the extract sample was analyzed by Thin Layer Chromatography (TLC). Cytotoxic activity using HeLa cells. Results: Phytochemical test of E. cottonii extracts showed the positive result for metabolite of flavonoid, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, n-hexane, chloroform, and ethyl acetate extracts of E. cottonii exhibited a strong cytotoxic activity against cervical HeLa cells with IC50 of 7.54 μg/mL, 5.73 μg /mL, 4.82 μg /mL and 4.34 μg / mL, respectively. Conclusion: The results suggest that macroalgae Eucheuma cottonii could be used as a new anti-cervical cancer’s candidate.
AB - Intoduction: Marine resource of macroalgae Eucheuma cottonii from East Lombok, Nusa Tenggara Barat, Indonesia, have potential as anti-cervical cancer agent. Objectives: Finding cytotoxic activity of Eucheuma cottonii hexane, ethylacetate, chloroform and ethanol extracts against cervical HeLa cells by MTT cell proliferation assay. Methods: The extracts was tested in phytochemical and cytotoxic activity test. Phytochemical test to identify composition of secondary metabolite such as flavonoid, alkaloid, saponins, tannin, triterpenoid, steroid and glycoside. The amount of substances contained in the extract sample was analyzed by Thin Layer Chromatography (TLC). Cytotoxic activity using HeLa cells. Results: Phytochemical test of E. cottonii extracts showed the positive result for metabolite of flavonoid, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, n-hexane, chloroform, and ethyl acetate extracts of E. cottonii exhibited a strong cytotoxic activity against cervical HeLa cells with IC50 of 7.54 μg/mL, 5.73 μg /mL, 4.82 μg /mL and 4.34 μg / mL, respectively. Conclusion: The results suggest that macroalgae Eucheuma cottonii could be used as a new anti-cervical cancer’s candidate.
KW - Anti-cervical cancer
KW - Cytotoxicity
KW - Eucheuma cottonii
KW - HeLa cell lines
KW - Phytochemical test
KW - Seaweed
UR - http://www.scopus.com/inward/record.url?scp=85051822943&partnerID=8YFLogxK
U2 - 10.5530/pj.2018.5.172
DO - 10.5530/pj.2018.5.172
M3 - Article
AN - SCOPUS:85051822943
SN - 0975-3575
VL - 10
SP - 1012
EP - 1017
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 5
ER -
