TY - JOUR
T1 - Phytochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.) leaves extract
AU - Rosmalena,
AU - Putri, Nabilla Aretharify
AU - Yazid, Fatmawaty
AU - Ambarwati, Neneng Siti Silfi
AU - Omar, Hanita
AU - Ahmad, Islamudin
N1 - Funding Information:
We would like to express our gratitude to International Indexed Publication Grant for Students Final Projects from the University of Indonesia 2018 which have supported this research.
Publisher Copyright:
© 2022 Journal of Advanced Pharmaceutical Technology & Research.
PY - 2022/4/1
Y1 - 2022/4/1
N2 - This current work aims to determine phytochemicals, in vitro radical scavenging, and in vivo oxidative stress reduction activities of peppermint (Mentha piperita L.) ethanolic extract (PEE). The Clule method was used to determine the phytochemical content. An in vitro antioxidant with radical scavenging activity was measured using 2,2-diphenyl-1-picrylhydrazyl. An in vivo antioxidant with oxidative stress reduction was carried out for 10 days on 25 male Sprague-Dawley rats (divided into five groups). Every day, each group was given positive control, negative control, 5, 10, and 20 mg/200 gr of body weight (BW) of the extract. The blood plasma was taken for malondialdehyde analysis. A phytochemical identification of PEE revealed more compounds, such as flavonoids, alkaloids, steroids, essential oils, and tannin. PEE exhibits significant in vitro radical scavenging activity, with an IC 50 value of 126.695 μg/mL. In the in vivo antioxidant with oxidative stress reduction experiments, 5 mg/200 gr BW was the most effective dose, as evidenced by a considerable drop in malondialdehyde level (0.312 nmol/mL) after and before treatment. In conclusion, PPE has the potential to be developed as a herbal antioxidant based on in vitro and in vivo test results.
AB - This current work aims to determine phytochemicals, in vitro radical scavenging, and in vivo oxidative stress reduction activities of peppermint (Mentha piperita L.) ethanolic extract (PEE). The Clule method was used to determine the phytochemical content. An in vitro antioxidant with radical scavenging activity was measured using 2,2-diphenyl-1-picrylhydrazyl. An in vivo antioxidant with oxidative stress reduction was carried out for 10 days on 25 male Sprague-Dawley rats (divided into five groups). Every day, each group was given positive control, negative control, 5, 10, and 20 mg/200 gr of body weight (BW) of the extract. The blood plasma was taken for malondialdehyde analysis. A phytochemical identification of PEE revealed more compounds, such as flavonoids, alkaloids, steroids, essential oils, and tannin. PEE exhibits significant in vitro radical scavenging activity, with an IC 50 value of 126.695 μg/mL. In the in vivo antioxidant with oxidative stress reduction experiments, 5 mg/200 gr BW was the most effective dose, as evidenced by a considerable drop in malondialdehyde level (0.312 nmol/mL) after and before treatment. In conclusion, PPE has the potential to be developed as a herbal antioxidant based on in vitro and in vivo test results.
KW - Antioxidant
KW - fifty percent inhibition concentration
KW - malondialdehyde level
KW - peppermint (Mentha piperita L.) extract
KW - phytochemical
UR - http://www.scopus.com/inward/record.url?scp=85129448461&partnerID=8YFLogxK
U2 - 10.4103/japtr.japtr_16_22
DO - 10.4103/japtr.japtr_16_22
M3 - Article
AN - SCOPUS:85129448461
SN - 0110-5558
VL - 13
SP - 133
EP - 137
JO - Journal of Advanced Pharmaceutical Technology and Research
JF - Journal of Advanced Pharmaceutical Technology and Research
IS - 2
ER -