TY - JOUR
T1 - Phytochemical content an in vitro toxicity of Glycine soja ethanol extract on the A549 Lung cancer line cell
AU - Amaani, R.
AU - Dwira, S.
N1 - Publisher Copyright:
© 2018 Institute of Physics Publishing. All rights reserved.
PY - 2018/9/7
Y1 - 2018/9/7
N2 - Lung cancer incidence is the highest of all cancers, and its management is still a challenge today. Natural anticancer compounds are being developed, one of which is derived from black soybeans. Black soybean (Glycine soja) contains bioactive compounds that are potential anticancer agents. The objective of this research was to determine the secondary metabolite content of black soybean ethanol extract and its toxicity to A549 lung cancer cells. Identification of the content of the extract was performed by thin layer chromatography (TLC) and phytochemical testing. Cytotoxicity testing was performed using an in vitro MTT-assay. The concentration tested were 6.25, 12.5, 25, 50, 100, 200, 400, and 800 μg/ml. The TLC results show that there were 6 compounds in the black soybean ethanol extract. Phytochemical test identified flavonoids, alkaloids, saponins, tannins, triterpenoids, and glycosides that have potential as anticancer agents. The extract exhibited moderate cytotoxic activity in A549 cells (IC50 = 114.5 μg/ml), and the highest extract concentration (800 μg/ml) gave the highest percentage inhibition (83.8%) and significantly different optical density than the other concentrations (p < 0.05). Black soybean ethanol extract has the potential to be developed as a lung anticancer agent.
AB - Lung cancer incidence is the highest of all cancers, and its management is still a challenge today. Natural anticancer compounds are being developed, one of which is derived from black soybeans. Black soybean (Glycine soja) contains bioactive compounds that are potential anticancer agents. The objective of this research was to determine the secondary metabolite content of black soybean ethanol extract and its toxicity to A549 lung cancer cells. Identification of the content of the extract was performed by thin layer chromatography (TLC) and phytochemical testing. Cytotoxicity testing was performed using an in vitro MTT-assay. The concentration tested were 6.25, 12.5, 25, 50, 100, 200, 400, and 800 μg/ml. The TLC results show that there were 6 compounds in the black soybean ethanol extract. Phytochemical test identified flavonoids, alkaloids, saponins, tannins, triterpenoids, and glycosides that have potential as anticancer agents. The extract exhibited moderate cytotoxic activity in A549 cells (IC50 = 114.5 μg/ml), and the highest extract concentration (800 μg/ml) gave the highest percentage inhibition (83.8%) and significantly different optical density than the other concentrations (p < 0.05). Black soybean ethanol extract has the potential to be developed as a lung anticancer agent.
UR - http://www.scopus.com/inward/record.url?scp=85054543485&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1073/3/032042
DO - 10.1088/1742-6596/1073/3/032042
M3 - Conference article
AN - SCOPUS:85054543485
SN - 1742-6588
VL - 1073
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 3
M1 - 032042
T2 - 2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018
Y2 - 18 July 2018 through 18 July 2018
ER -