TY - JOUR
T1 - Phytochemical Constituents, Anti-Diabetic and Antioxidant Activities of Methanol Extracts of Diospyros malabarica (Desr.) Kostel Leaves and Stem Bark
AU - Dharmastuti, Wening
AU - Elya, Berna
AU - Hanafi, Muhammad
AU - Lotulung, Puspa D.N.
N1 - Publisher Copyright:
© 2024 Dharmastuti et al.
PY - 2024/5/30
Y1 - 2024/5/30
N2 - Diospyros malabarica (Desr.) Kostel is widely used in traditional medicine as an antioxidant and anti-diabetic agent. This study aims to evaluate the phytochemical constituents, the antioxidant and anti-diabetic activities of the methanol leaf extract (MLE) and methanol stem bark extract (MBE) of Diospyros malabarica. The extracts were obtained by Ultrasound-Assisted Extraction (UAE). Phytochemical screening was done using standard methods. The total phenolic content (TPC) and total flavonoid content (TFC) were determined using the Folin-Ciocalteau and Aluminium chloride colorimetric methods, respectively. The antidiabetic activity was assessed using α-glucosidase and dipeptidyl peptidase-4 (DPP-4) inhibitory assays. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and Ferric Reducing Antioxidant Power (FRAP) assays. The results showed that the TPC (621.31 ± 0.74 mgGAE/g) and TFC (32.86 ± 0.31 mgQE/g) of MBE are 14 times higher than that of MLE with TPC and TFC of 42.83 ± 0.15 mgGAE/g, and 2.37 ± 0.02 mgQE/g, respectively. MBE also showed higher antioxidant and anti-diabetic activities than MLE. The IC50 values of MBE for DPPH radical scavenging, ABTS radical scavenging, and FRAP activities were 8.04 ± 0.05 µg/mL, 2.61 ± 0.03 µg/mL, and 6803.86 µMFSE/g, respectively. For the anti-diabetic activity, MBE had IC50 values of 14.36 ± 0.21 µg/mL, and 205.39 ± 2.94 µg/mL for α-glucosidase, and DPP-4 inhibitory activities, respectively. Therefore, the stem bark of D. malabarica has better anti-diabetic and antioxidant activities than the leaves, and thus has a potential for use as an antioxidant and anti-diabetic agent.
AB - Diospyros malabarica (Desr.) Kostel is widely used in traditional medicine as an antioxidant and anti-diabetic agent. This study aims to evaluate the phytochemical constituents, the antioxidant and anti-diabetic activities of the methanol leaf extract (MLE) and methanol stem bark extract (MBE) of Diospyros malabarica. The extracts were obtained by Ultrasound-Assisted Extraction (UAE). Phytochemical screening was done using standard methods. The total phenolic content (TPC) and total flavonoid content (TFC) were determined using the Folin-Ciocalteau and Aluminium chloride colorimetric methods, respectively. The antidiabetic activity was assessed using α-glucosidase and dipeptidyl peptidase-4 (DPP-4) inhibitory assays. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and Ferric Reducing Antioxidant Power (FRAP) assays. The results showed that the TPC (621.31 ± 0.74 mgGAE/g) and TFC (32.86 ± 0.31 mgQE/g) of MBE are 14 times higher than that of MLE with TPC and TFC of 42.83 ± 0.15 mgGAE/g, and 2.37 ± 0.02 mgQE/g, respectively. MBE also showed higher antioxidant and anti-diabetic activities than MLE. The IC50 values of MBE for DPPH radical scavenging, ABTS radical scavenging, and FRAP activities were 8.04 ± 0.05 µg/mL, 2.61 ± 0.03 µg/mL, and 6803.86 µMFSE/g, respectively. For the anti-diabetic activity, MBE had IC50 values of 14.36 ± 0.21 µg/mL, and 205.39 ± 2.94 µg/mL for α-glucosidase, and DPP-4 inhibitory activities, respectively. Therefore, the stem bark of D. malabarica has better anti-diabetic and antioxidant activities than the leaves, and thus has a potential for use as an antioxidant and anti-diabetic agent.
KW - Alpha-glucosidase
KW - Anti-diabetic
KW - Antioxidant
KW - Diospyros malabarica (Desr.) Kostel
KW - DPP-4
UR - http://www.scopus.com/inward/record.url?scp=85195969738&partnerID=8YFLogxK
U2 - 10.26538/tjnpr/v8i5.29
DO - 10.26538/tjnpr/v8i5.29
M3 - Article
AN - SCOPUS:85195969738
SN - 2616-0684
VL - 8
SP - 7249
EP - 7258
JO - Tropical Journal of Natural Product Research
JF - Tropical Journal of Natural Product Research
IS - 5
ER -