TY - JOUR
T1 - Phytochemical composition and anticancer activity of seaweeds Ulva lactuca and Eucheuma cottonii against breast MCF-7 and colon HCT-116 cells
AU - Arsianti, Ade
AU - Fadilah, null
AU - Fatmawaty, null
AU - Wibisono, Lies Kurniatis
AU - Kusmardi, Suid
AU - Azizah, Norma Nur
AU - Putrianingsih, Rista
AU - Murniasih, Tutik
AU - Rasyid, Abdullah
AU - Pangestuti, Ratih
N1 - Publisher Copyright:
© 2016 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
PY - 2016/11
Y1 - 2016/11
N2 - Objective: This study is aimed to develop marine resources, which is focused on the determination of phytochemical composition and exploration of seaweeds Ulva lactuca and Eucheuma cottonii, as a potential antibreast cancer and anticolorectal cancer agents. Methods: Seaweeds U. lactuca collected from Parangtritis beach, Yogyakarta, Central Java, Indonesia, whereas E. cottonii collected from Salemo Island, South Sulawesi, Indonesia. Seaweeds U. lactuca and E. cottonii were macerated in organic solvents, n-hexane, chloroform, ethyl acetate, and ethanol, respectively. After maceration for 3 days, the mixture was filtered; the filtrate was concentrated by rotary evaporator. The concentrated extract of n-hexane, ethyl acetate, ethanol, and chloroform was then analyzed by thin layer chromatography. Phytochemical test of the concentrated extract was conducted to identify the metabolites containing in the seaweeds. Furthermore, the cytotoxic activity of the n-hexane, ethyl acetate, ethanol, and chloroform extract of U. lactuca and E. cotonii were evaluated as a growth inhibitor of breast MCF-7 and colorectal HCT-116 cancer cells by MTT cell proliferation assay. Results: Phytochemical test for the concentrated extracts of U. Lactuca showed the positive result for metabolites of steroids, glycosides, flavonoids, and tannins, whereas the concentrated extracts of E. cottonii showed the positive result for metabolites of steroids, glycosides, and flavonoid. Both concentrated extracts of U. lactuca and E. cotonii exhibited anticancer activity against breast MCF-7 and colorectal HCT-116 cells with IC50 ranging from 21 µg/mL to 99 µg/mL. Conclusion: Our results clearly demonstrate seaweeds U. Lactuca and E. cotonii as promising candidates for the new antibreast and anticolorectal cancer agents.
AB - Objective: This study is aimed to develop marine resources, which is focused on the determination of phytochemical composition and exploration of seaweeds Ulva lactuca and Eucheuma cottonii, as a potential antibreast cancer and anticolorectal cancer agents. Methods: Seaweeds U. lactuca collected from Parangtritis beach, Yogyakarta, Central Java, Indonesia, whereas E. cottonii collected from Salemo Island, South Sulawesi, Indonesia. Seaweeds U. lactuca and E. cottonii were macerated in organic solvents, n-hexane, chloroform, ethyl acetate, and ethanol, respectively. After maceration for 3 days, the mixture was filtered; the filtrate was concentrated by rotary evaporator. The concentrated extract of n-hexane, ethyl acetate, ethanol, and chloroform was then analyzed by thin layer chromatography. Phytochemical test of the concentrated extract was conducted to identify the metabolites containing in the seaweeds. Furthermore, the cytotoxic activity of the n-hexane, ethyl acetate, ethanol, and chloroform extract of U. lactuca and E. cotonii were evaluated as a growth inhibitor of breast MCF-7 and colorectal HCT-116 cancer cells by MTT cell proliferation assay. Results: Phytochemical test for the concentrated extracts of U. Lactuca showed the positive result for metabolites of steroids, glycosides, flavonoids, and tannins, whereas the concentrated extracts of E. cottonii showed the positive result for metabolites of steroids, glycosides, and flavonoid. Both concentrated extracts of U. lactuca and E. cotonii exhibited anticancer activity against breast MCF-7 and colorectal HCT-116 cells with IC50 ranging from 21 µg/mL to 99 µg/mL. Conclusion: Our results clearly demonstrate seaweeds U. Lactuca and E. cotonii as promising candidates for the new antibreast and anticolorectal cancer agents.
KW - Anticancer
KW - Breast MCF-7
KW - Colorectal HCT-116
KW - Eucheuma cottonii
KW - Phytochemistry
KW - Ulva lactuca
UR - http://www.scopus.com/inward/record.url?scp=84994895374&partnerID=8YFLogxK
U2 - 10.22159/ajpcr.2016.v9i6.13798
DO - 10.22159/ajpcr.2016.v9i6.13798
M3 - Article
AN - SCOPUS:84994895374
SN - 0974-2441
VL - 9
SP - 115
EP - 119
JO - Asian Journal of Pharmaceutical and Clinical Research
JF - Asian Journal of Pharmaceutical and Clinical Research
IS - 6
ER -