TY - JOUR
T1 - Phytochemical Analysis and Antifungal Activity of Brunei Propolis Against Candida sp. and Cryptococcus sp.
AU - Sabrina, Geani
AU - Adawiyah, Robiatul
AU - Usman, Anwar
AU - Mayhana, Shuffa Chilla
AU - Sihotang, Diandra Imanuella Zilver
AU - Sahlan, Muhamad
N1 - Funding Information:
LCMS/MS analysis was performed at the Advanced Research Laboratory of IPB University, and the Antifungal test was performed at Laboratory of the Department of Parasitology Faculty of Medicine Universitas Indonesia is gratefully acknowledged.
Publisher Copyright:
© 2022,International Journal of Technology.All Rights Reserved.
PY - 2022
Y1 - 2022
N2 - The propolis used in this study is propolis from Brunei Darussalam, including G. thoracica, H. itama, and T. binghami. This study focuses on evaluating phytochemicals, including the total content of polyphenols and flavonoids, marker compounds, and the anti-fungal activity of propolis Brunei. Until now, research on the compounds contained in propolis is still being carried out. However, the literature on the chemical compound of Brunei propolis is still limited. The results of the research on the content of Brunei propolis using LC-MS/MS found as many as 21 chemical compounds and three marker compounds, namely maslinic acid, D-(-) Mannitol, and 18-β-Glycyrrhetinic acid. The total content of flavonoid and polyphenolic propolis in Brunei was obtained using quercetin as a flavonoid standard and gallic acid as a polyphenol standard. In Brunei propolis, the total flavonoid content was higher than the total polyphenol content. Where the total polyphenol content of propolis G. thoracica, H. itama, and T. binghami were 78.79±17.06; 70.51±12.93 and 16.37±0.53 (mgGAE/g). while the total flavonoid content was 19.30±1.99; 101.10±6.26 and 61.63±4.53 (mg QE/g). The antifungal activity was carried out by agar diffusion and microdilution methods. Brunei propolis extract showed antifungal activity against Cryptococcus and C. Albicans, whereas propolis Brunei extract showed anti-fungal activity with intermediate resistance to both fungi.
AB - The propolis used in this study is propolis from Brunei Darussalam, including G. thoracica, H. itama, and T. binghami. This study focuses on evaluating phytochemicals, including the total content of polyphenols and flavonoids, marker compounds, and the anti-fungal activity of propolis Brunei. Until now, research on the compounds contained in propolis is still being carried out. However, the literature on the chemical compound of Brunei propolis is still limited. The results of the research on the content of Brunei propolis using LC-MS/MS found as many as 21 chemical compounds and three marker compounds, namely maslinic acid, D-(-) Mannitol, and 18-β-Glycyrrhetinic acid. The total content of flavonoid and polyphenolic propolis in Brunei was obtained using quercetin as a flavonoid standard and gallic acid as a polyphenol standard. In Brunei propolis, the total flavonoid content was higher than the total polyphenol content. Where the total polyphenol content of propolis G. thoracica, H. itama, and T. binghami were 78.79±17.06; 70.51±12.93 and 16.37±0.53 (mgGAE/g). while the total flavonoid content was 19.30±1.99; 101.10±6.26 and 61.63±4.53 (mg QE/g). The antifungal activity was carried out by agar diffusion and microdilution methods. Brunei propolis extract showed antifungal activity against Cryptococcus and C. Albicans, whereas propolis Brunei extract showed anti-fungal activity with intermediate resistance to both fungi.
KW - Anti-fungal
KW - Brunei propolis
KW - Lc-ms/ms
KW - Total flavonoid content
KW - Total polyphenol content
UR - http://www.scopus.com/inward/record.url?scp=85146314299&partnerID=8YFLogxK
U2 - 10.14716/ijtech.v13i8.6135
DO - 10.14716/ijtech.v13i8.6135
M3 - Article
AN - SCOPUS:85146314299
SN - 2086-9614
VL - 13
SP - 1640
EP - 1650
JO - International Journal of Technology
JF - International Journal of Technology
IS - 8
ER -