TY - JOUR
T1 - Phytochemical analysis and anticancer activity of seaweed gracilaria verrucosa against colorectal HCT-116 cells
AU - Kurniasari, Kartika D.W.I.
AU - Arsianti, Ade
AU - Nugrahayning Aziza, Yully Astika
AU - Dyahningrum Mandasari, Baiq Kirana
AU - Masita, Riathul
AU - Ruhama Zulfa, Futihati
AU - Kusumaning Dewi, Micheylla
AU - Zahira Zagloel, Cut Raisya
AU - Azizah, Norma N.U.R.
AU - Putrianingsih, Rista
N1 - Publisher Copyright:
© 2018 Oriental Scientific Publishing Company. All Rights Reserved.
PY - 2018
Y1 - 2018
N2 - In this research, we develop Indonesia marine resource of seaweed Gracilaria verrucosa as an anti-colorectal cancer agent. Seaweed Gracilaria verrucosa which was collected from east Lombok beach, Nusa Tenggara Barat, Indonesia, were extracted into four different organic solvents, that is n-hexane, ethylacetate, chloroform and ethanol.The extracts were analyzed by Phytochemical test and Thin Layer Chromatography (TLC). Subsequently, anticancer activity of n-hexane, ethyl acetate, chloroform and ethanol extracts of Gracilaria verrucosa were evaluated against colorectal HCT-116 cells by MTT cell proliferation assay. Based on Phytochemical analysis, the extracts of Gracilaria verrucosa containing secondary metabolite of saponin, while TLC analysis indicated that the extracts were composed by five chemical compounds. Among four concentrated extracts of Gracilaria verrucosa, ethanolic extract showed the strongest anticancer activity against colorectal HCT-116 cells with IC50 of 43.9 μg/mL.Ethanolic extract of seaweed Gracilaria verrucosa is potential to be further developed as a promising anti-colorectal cancer agents.
AB - In this research, we develop Indonesia marine resource of seaweed Gracilaria verrucosa as an anti-colorectal cancer agent. Seaweed Gracilaria verrucosa which was collected from east Lombok beach, Nusa Tenggara Barat, Indonesia, were extracted into four different organic solvents, that is n-hexane, ethylacetate, chloroform and ethanol.The extracts were analyzed by Phytochemical test and Thin Layer Chromatography (TLC). Subsequently, anticancer activity of n-hexane, ethyl acetate, chloroform and ethanol extracts of Gracilaria verrucosa were evaluated against colorectal HCT-116 cells by MTT cell proliferation assay. Based on Phytochemical analysis, the extracts of Gracilaria verrucosa containing secondary metabolite of saponin, while TLC analysis indicated that the extracts were composed by five chemical compounds. Among four concentrated extracts of Gracilaria verrucosa, ethanolic extract showed the strongest anticancer activity against colorectal HCT-116 cells with IC50 of 43.9 μg/mL.Ethanolic extract of seaweed Gracilaria verrucosa is potential to be further developed as a promising anti-colorectal cancer agents.
KW - Anticancer activity
KW - Colorectal HCT-116 cells
KW - Gracillaria verrucosa
KW - Phytochemistry
UR - http://www.scopus.com/inward/record.url?scp=85049744567&partnerID=8YFLogxK
U2 - 10.13005/ojc/340308
DO - 10.13005/ojc/340308
M3 - Article
AN - SCOPUS:85049744567
SN - 0970-020X
VL - 34
SP - 1257
EP - 1262
JO - Oriental Journal of Chemistry
JF - Oriental Journal of Chemistry
IS - 3
ER -