Phytochemical analysis and anticancer activity of seaweed gracilaria verrucosa against colorectal HCT-116 cells

Kartika D.W.I. Kurniasari, Ade Arsianti, Yully Astika Nugrahayning Aziza, Baiq Kirana Dyahningrum Mandasari, Riathul Masita, Futihati Ruhama Zulfa, Micheylla Kusumaning Dewi, Cut Raisya Zahira Zagloel, Norma N.U.R. Azizah, Rista Putrianingsih

Research output: Contribution to journalArticlepeer-review

Abstract

In this research, we develop Indonesia marine resource of seaweed Gracilaria verrucosa as an anti-colorectal cancer agent. Seaweed Gracilaria verrucosa which was collected from east Lombok beach, Nusa Tenggara Barat, Indonesia, were extracted into four different organic solvents, that is n-hexane, ethylacetate, chloroform and ethanol.The extracts were analyzed by Phytochemical test and Thin Layer Chromatography (TLC). Subsequently, anticancer activity of n-hexane, ethyl acetate, chloroform and ethanol extracts of Gracilaria verrucosa were evaluated against colorectal HCT-116 cells by MTT cell proliferation assay. Based on Phytochemical analysis, the extracts of Gracilaria verrucosa containing secondary metabolite of saponin, while TLC analysis indicated that the extracts were composed by five chemical compounds. Among four concentrated extracts of Gracilaria verrucosa, ethanolic extract showed the strongest anticancer activity against colorectal HCT-116 cells with IC50 of 43.9 μg/mL.Ethanolic extract of seaweed Gracilaria verrucosa is potential to be further developed as a promising anti-colorectal cancer agents.

Original languageEnglish
Pages (from-to)1257-1262
Number of pages6
JournalOriental Journal of Chemistry
Volume34
Issue number3
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Anticancer activity
  • Colorectal HCT-116 cells
  • Gracillaria verrucosa
  • Phytochemistry

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