TY - GEN
T1 - Phytochemical analysis and anticancer activity of seaweed Eucheuma Sp. against colon HCT-116 cells
AU - Subroto, Priscilla Aya Maheswari
AU - Arsianti, Ade
AU - Putri, Trivani
AU - Lesmana, Elvira
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/4/9
Y1 - 2019/4/9
N2 -
Seaweed macroalgae is one of marine natural product which shows a potent anticancer activity. In this work, we developed seaweed Eucheuma Sp. collected from east Lombok beach, Nusa Tenggara Barat, Indonesia, as potential anti-colorectal cancer agents. Seaweed macroalgae Eucheuma Sp. were extracted in four kind of organic solvents of hexane, ethylacetate, chloroform and ethanol, respectively. The extracts were tested for its phytochemical composition, and were then analyzed by Thin Layer Chromatography (TLC). Furthermore, anticancer activity of hexane, ethyl acetate, chloroform and ethanol extracts of Eucheuma Sp. were examined against colon HCT-116 cells by MTT cell proliferation assay. Phytochemical test of the extracts of Eucheuma Sp. showed positive result for metabolite of flavonoid, triterpenoid, alkaloid and tannin, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, hexane, chloroform, and ethyl acetate extracts of Eucheuma Sp. exhibited a good anticancer activity against colon HCT-116 cells with IC
50
value ranging from 16.82μg/mL to 40.45μg/mL. The results suggest that seaweed Eucheuma Sp. could be further developed as a candidate for the new anti-colorectal cancer agents.
AB -
Seaweed macroalgae is one of marine natural product which shows a potent anticancer activity. In this work, we developed seaweed Eucheuma Sp. collected from east Lombok beach, Nusa Tenggara Barat, Indonesia, as potential anti-colorectal cancer agents. Seaweed macroalgae Eucheuma Sp. were extracted in four kind of organic solvents of hexane, ethylacetate, chloroform and ethanol, respectively. The extracts were tested for its phytochemical composition, and were then analyzed by Thin Layer Chromatography (TLC). Furthermore, anticancer activity of hexane, ethyl acetate, chloroform and ethanol extracts of Eucheuma Sp. were examined against colon HCT-116 cells by MTT cell proliferation assay. Phytochemical test of the extracts of Eucheuma Sp. showed positive result for metabolite of flavonoid, triterpenoid, alkaloid and tannin, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, hexane, chloroform, and ethyl acetate extracts of Eucheuma Sp. exhibited a good anticancer activity against colon HCT-116 cells with IC
50
value ranging from 16.82μg/mL to 40.45μg/mL. The results suggest that seaweed Eucheuma Sp. could be further developed as a candidate for the new anti-colorectal cancer agents.
KW - anticancer activity
KW - Eucheuma Sp.
KW - HCT-116 cells
KW - MTT assay
KW - phytochemistry
KW - seaweed
UR - http://www.scopus.com/inward/record.url?scp=85064807169&partnerID=8YFLogxK
U2 - 10.1063/1.5096719
DO - 10.1063/1.5096719
M3 - Conference contribution
AN - SCOPUS:85064807169
T3 - AIP Conference Proceedings
BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Wulan, Praswasti P.D.K.
A2 - Gozan, Misri
A2 - Astutiningsih, Sotya
A2 - Ramahdita, Ghiska
A2 - Dhelika, Radon
A2 - Kreshanti, Prasetyanugraheni
PB - American Institute of Physics Inc.
T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Y2 - 6 August 2018 through 8 August 2018
ER -
