Phytochemical analysis and anticancer activity of seaweed Eucheuma Sp. against colon HCT-116 cells

Priscilla Aya Maheswari Subroto, Ade Arsianti, Trivani Putri, Elvira Lesmana

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Seaweed macroalgae is one of marine natural product which shows a potent anticancer activity. In this work, we developed seaweed Eucheuma Sp. collected from east Lombok beach, Nusa Tenggara Barat, Indonesia, as potential anti-colorectal cancer agents. Seaweed macroalgae Eucheuma Sp. were extracted in four kind of organic solvents of hexane, ethylacetate, chloroform and ethanol, respectively. The extracts were tested for its phytochemical composition, and were then analyzed by Thin Layer Chromatography (TLC). Furthermore, anticancer activity of hexane, ethyl acetate, chloroform and ethanol extracts of Eucheuma Sp. were examined against colon HCT-116 cells by MTT cell proliferation assay. Phytochemical test of the extracts of Eucheuma Sp. showed positive result for metabolite of flavonoid, triterpenoid, alkaloid and tannin, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, hexane, chloroform, and ethyl acetate extracts of Eucheuma Sp. exhibited a good anticancer activity against colon HCT-116 cells with IC 50 value ranging from 16.82μg/mL to 40.45μg/mL. The results suggest that seaweed Eucheuma Sp. could be further developed as a candidate for the new anti-colorectal cancer agents.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
CountryIndonesia
CityJakarta
Period6/08/188/08/18

Fingerprint

seaweeds
chloroform
thin layer chromatography
ethyl alcohol
cells
acetates
cancer
Indonesia
chemical compounds
alkaloids
beaches
metabolites
products

Keywords

  • anticancer activity
  • Eucheuma Sp.
  • HCT-116 cells
  • MTT assay
  • phytochemistry
  • seaweed

Cite this

Subroto, P. A. M., Arsianti, A., Putri, T., & Lesmana, E. (2019). Phytochemical analysis and anticancer activity of seaweed Eucheuma Sp. against colon HCT-116 cells. In P. P. D. K. Wulan, M. Gozan, S. Astutiningsih, G. Ramahdita, R. Dhelika, & P. Kreshanti (Eds.), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018 [030015] (AIP Conference Proceedings; Vol. 2092). American Institute of Physics Inc.. https://doi.org/10.1063/1.5096719
Subroto, Priscilla Aya Maheswari ; Arsianti, Ade ; Putri, Trivani ; Lesmana, Elvira. / Phytochemical analysis and anticancer activity of seaweed Eucheuma Sp. against colon HCT-116 cells. 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. editor / Praswasti P.D.K. Wulan ; Misri Gozan ; Sotya Astutiningsih ; Ghiska Ramahdita ; Radon Dhelika ; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).
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abstract = "Seaweed macroalgae is one of marine natural product which shows a potent anticancer activity. In this work, we developed seaweed Eucheuma Sp. collected from east Lombok beach, Nusa Tenggara Barat, Indonesia, as potential anti-colorectal cancer agents. Seaweed macroalgae Eucheuma Sp. were extracted in four kind of organic solvents of hexane, ethylacetate, chloroform and ethanol, respectively. The extracts were tested for its phytochemical composition, and were then analyzed by Thin Layer Chromatography (TLC). Furthermore, anticancer activity of hexane, ethyl acetate, chloroform and ethanol extracts of Eucheuma Sp. were examined against colon HCT-116 cells by MTT cell proliferation assay. Phytochemical test of the extracts of Eucheuma Sp. showed positive result for metabolite of flavonoid, triterpenoid, alkaloid and tannin, whereas the TLC analysis revealed that the extracts containing five chemical compounds. Ethanol, hexane, chloroform, and ethyl acetate extracts of Eucheuma Sp. exhibited a good anticancer activity against colon HCT-116 cells with IC 50 value ranging from 16.82μg/mL to 40.45μg/mL. The results suggest that seaweed Eucheuma Sp. could be further developed as a candidate for the new anti-colorectal cancer agents.",
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Subroto, PAM, Arsianti, A, Putri, T & Lesmana, E 2019, Phytochemical analysis and anticancer activity of seaweed Eucheuma Sp. against colon HCT-116 cells. in PPDK Wulan, M Gozan, S Astutiningsih, G Ramahdita, R Dhelika & P Kreshanti (eds), 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018., 030015, AIP Conference Proceedings, vol. 2092, American Institute of Physics Inc., 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018, Jakarta, Indonesia, 6/08/18. https://doi.org/10.1063/1.5096719

Phytochemical analysis and anticancer activity of seaweed Eucheuma Sp. against colon HCT-116 cells. / Subroto, Priscilla Aya Maheswari; Arsianti, Ade; Putri, Trivani; Lesmana, Elvira.

3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. ed. / Praswasti P.D.K. Wulan; Misri Gozan; Sotya Astutiningsih; Ghiska Ramahdita; Radon Dhelika; Prasetyanugraheni Kreshanti. American Institute of Physics Inc., 2019. 030015 (AIP Conference Proceedings; Vol. 2092).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Subroto PAM, Arsianti A, Putri T, Lesmana E. Phytochemical analysis and anticancer activity of seaweed Eucheuma Sp. against colon HCT-116 cells. In Wulan PPDK, Gozan M, Astutiningsih S, Ramahdita G, Dhelika R, Kreshanti P, editors, 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2018. American Institute of Physics Inc. 2019. 030015. (AIP Conference Proceedings). https://doi.org/10.1063/1.5096719