PHYSIOLOGY AND THE EFFECT OF SOME DRUGS ON OSTEOCLAST

The osteoclast cellular process is essential for bone growth, remodeling, and repair, and is, under normal conditions, tightly coupled to the process of bone formation by the osteoblast. It is the balance that determines skeletal mass and shape at any point in time. Some drugs effect the osteoclast formation and differentiation. Biphosphonate and non-steroid anti-inflammatory drugs (NSAIDs_ are known to reduce the resorption process of the bone in vitro. We worked on those two different drugs to see their effects and influences on bone remodeling, especially bone resoprtion using a bone marrow culture system. This culture system was found to be suitable for the recruitment and differentiation process of osteoclast-like cells from their progenitors. Biphosphonate and two NSAIDs that were used in this study using 1,25(OH)2D3 as an osteoclast stimulani, proved to be very powerful inhibitor of bone resorption. Their mechanisms of action of bone resorption inhibition using bone marrow culture system are almost similar, which is during the later stage of differentiation. The improvement of our means of therapeutic intervention with antiresorptive agents will depend on our better understanding of the molecular basis of bone resorption.