Physical and chemical stability test of neem oil cream (Azadirachta indica) using high-performance liquid chromatography

Febrina Amelia Saputri, Patihul Husni, Norisca Aliza Putriana

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Objective: This study aims to examine the physical and chemical stability of neem oil cream. Methods: Physical stability was conducted by storing the cream at room temperature (25±2 °C/65 %RH±5 %RH) and high temperature (40±2 °C/75 % RH±5 % RH) for 3 mo. HPLC method using Dionex with UV detection at 219 nm, Shodex (C-18) HPLC packed column (4.6 mmID x 250 mmL), acetonitrile: water [30:70] as mobile phase, 10 min isocratic elution with a flow rate of 1.0 ml/min with volume injection 20 μL was validated then was carried out to measure azadirachtin levels in neem oil cream. The chemical stability of azadirachtin in the cream was determined for 90 days by using this validated method. Results: The neem oil cream was physically stable. The HPLC method of azadirachtin meets all the validation parameters and can be used to analyze the chemical stability of azadirachtin in neem oil cream. Neem oil cream was stable for 4 w at 25 °C and for 1 w at 40 °C. Conclusion: The neem oil cream was either physically or chemically stable for 4 weeks at 25oC and 1 week at 40oC.

Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalInternational Journal of Applied Pharmaceutics
Volume13
Issue number4
DOIs
Publication statusPublished - 1 Jul 2021

Keywords

  • Azadirachtin
  • Chemical stability
  • High-performance liquid chromatography
  • Neem oil cream
  • Physical stability

Fingerprint

Dive into the research topics of 'Physical and chemical stability test of neem oil cream (Azadirachta indica) using high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this