Pharmacokinetic profile of metformin hydrochloride in dried blood spot of healthy subjects using high-performance liquid chromatography-photodiode array

Objective: This study aimed to analyze the metformin hydrochloride in dried blood spot (DBS) sample and evaluate a pharmacokinetic profile in six healthy subjects who administered 850-mg metformin hydrochloride in tablet using high-performance liquid chromatography-photodiode array (HPLC-PDA). Methods: Metformin analysis was performed on data of six healthy subjects who administered 850 mg of metformin hydrochloride as tablets. Blood samples were taken at 12-time points at intervals of up to 12 h, and data were analyzed using HPLC-PDA. Results: A linear calibration curve was obtained in the range of 25-5000 ng/mL, with r=0.9990. Pharmacokinetic profiles of metformin were obtained using DBS samples from six healthy participants, with a C_max of 347.3-416.22 ng/mL. The average t_max and t_1/2 were obtained at 3 and 2 h, respectively, and the area under the curve (AUC)_0-t/AUC_0-8 ratio was >80% for all subjects. Conclusion: The DBS biosampling technique can be developed for application in pharmacokinetic study of metformin HCl.