Pharmacokinetic profile of metformin hydrochloride in dried blood spot of healthy subjects using high-performance liquid chromatography-photodiode array

Yahdiana Harahap, Citra Dara Malia, Sunarsih

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: This study aimed to analyze the metformin hydrochloride in dried blood spot (DBS) sample and evaluate a pharmacokinetic profile in six healthy subjects who administered 850-mg metformin hydrochloride in tablet using high-performance liquid chromatography-photodiode array (HPLC-PDA). Methods: Metformin analysis was performed on data of six healthy subjects who administered 850 mg of metformin hydrochloride as tablets. Blood samples were taken at 12-time points at intervals of up to 12 h, and data were analyzed using HPLC-PDA. Results: A linear calibration curve was obtained in the range of 25-5000 ng/mL, with r=0.9990. Pharmacokinetic profiles of metformin were obtained using DBS samples from six healthy participants, with a Cmax of 347.3-416.22 ng/mL. The average tmax and t1/2 were obtained at 3 and 2 h, respectively, and the area under the curve (AUC)0-t/AUC0-8 ratio was >80% for all subjects. Conclusion: The DBS biosampling technique can be developed for application in pharmacokinetic study of metformin HCl.

Original languageEnglish
Pages (from-to)354-357
Number of pages4
JournalInternational Journal of Applied Pharmaceutics
Volume10
Issue numberSpecial Issue 1
DOIs
Publication statusPublished - 1 Dec 2018

Keywords

  • Dried blood spot
  • High-performance liquid chromatography
  • Metformin hydrochloride
  • Pharmacokinetic profile
  • Validation

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