TY - JOUR
T1 - Pharmacognostic and Cytotoxicity Evaluation of Indonesia Native Plant of Piper acre Blume Leaves (Piperaceae)
AU - Ahmad, Islamudin
AU - Ambarwati, Neneng Silfi Siti
AU - Arifuddin, Muhammad
AU - Rijai, Laode
AU - Mun'im, Abdul
N1 - Publisher Copyright:
© 2017 Phcog.Net.
PY - 2017
Y1 - 2017
N2 - Piper acre Blume (Piperaceae) is an Indonesia native plant that is used as a traditional medicine and commonly known as 'Sirih Hitam' or 'Sirih Hutan'. The scientific data from the plant is still very limited. The aims of the present study was to know a pharmacognostic, and cytotoxicity of P. acre Blume leaf. The macroscopic characters showed that the leaves have a characteristic odor, heart-shaped, margin was finely flat, venation was curved, the upper surface was dark green and slippery while the lower surface was pale green and soft. The microscopic characters revealed upper and lower epidermis cells, glandular trichomes, concentric vascular bundle, paracytic stomata. The fluorescence characteristics showed a variety of chemical constituents present in the plant materials. The preliminary phytochemical investigation of various extracts showed the presence of alkaloid, flavonoid, saponin, terpenoid, tannin, carotenoid, and steroid. The physicochemical properties (yield, color, consistency) of the n-hexane, ethyl acetate, and ethanol extract were 31.57% w/w (green concentrated solid), 13,59% w/w (green concentrated solid), and 16.35% w/w (brownish green liquid). Based on the cytotoxicity using brine shrimp lethality assay of the n-hexane extract (2.46 μg/ml), ethyl acetate extract (0.925 μg/ml), and ethanol extract (5.741 μg/ml) showed that the extract had the cytotoxic activity.
AB - Piper acre Blume (Piperaceae) is an Indonesia native plant that is used as a traditional medicine and commonly known as 'Sirih Hitam' or 'Sirih Hutan'. The scientific data from the plant is still very limited. The aims of the present study was to know a pharmacognostic, and cytotoxicity of P. acre Blume leaf. The macroscopic characters showed that the leaves have a characteristic odor, heart-shaped, margin was finely flat, venation was curved, the upper surface was dark green and slippery while the lower surface was pale green and soft. The microscopic characters revealed upper and lower epidermis cells, glandular trichomes, concentric vascular bundle, paracytic stomata. The fluorescence characteristics showed a variety of chemical constituents present in the plant materials. The preliminary phytochemical investigation of various extracts showed the presence of alkaloid, flavonoid, saponin, terpenoid, tannin, carotenoid, and steroid. The physicochemical properties (yield, color, consistency) of the n-hexane, ethyl acetate, and ethanol extract were 31.57% w/w (green concentrated solid), 13,59% w/w (green concentrated solid), and 16.35% w/w (brownish green liquid). Based on the cytotoxicity using brine shrimp lethality assay of the n-hexane extract (2.46 μg/ml), ethyl acetate extract (0.925 μg/ml), and ethanol extract (5.741 μg/ml) showed that the extract had the cytotoxic activity.
KW - Cytotoxicity
KW - Pharmacognostic
KW - Physicochemical
KW - Piper acre blume
KW - Preliminary phytochemical
UR - http://www.scopus.com/inward/record.url?scp=85018566056&partnerID=8YFLogxK
U2 - 10.5530/pj.2017.3.68
DO - 10.5530/pj.2017.3.68
M3 - Article
AN - SCOPUS:85018566056
SN - 0975-3575
VL - 9
SP - 400
EP - 404
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 3
ER -