Performance of TDR-300B and VITEK®2 for the identification of Pseudomonas aeruginosa in comparison with VITEK®-MS

Lucky Hartati Moehario, Enty Tjoa, Hans Putranata, Shikha Joon, Daniel Edbert, Thomas Robertus

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: Automated systems are needed for the rapid and accurate diagnosis of Pseudomonas-associated nosocomial infections among critically ill patients admitted to the intensive care unit. We assessed the performance of TDR-300B and VITEK®2 for the identification of P. aeruginosa using VITEK®-MS as the gold standard. Methods: This analytical study employed a cross-sectional approach. First, 44 clinical isolates of P. aeruginosa were collected and refreshed. Next, a single colony of oxidase-positive, gram-negative rods (30 samples) was inoculated into a TDR-300B NF-64 card and VITEK®2 GN cassette for each isolate. Finally, bacterial identification was performed using VITEK®-MS for comparative analysis. Results: Compared with the results for VITEK®-MS, the congruence rates for TDR-300B and VITEK®2 were 80.76% (21/26) and 92.30% (24/26), respectively. Further, high sensitivity was observed for TDR-300B and VITEK®2 (95.45% and 100%, respectively). In addition, TDR-300B had a lower positive predictive value and accuracy than VITEK®2, albeit without significance. Conclusions: Conclusively, there were no significant differences regarding the diagnostic efficiency of TDR-300B and VITEK®2 for P. aeruginosa.

Original languageEnglish
JournalJournal of International Medical Research
Volume49
Issue number2
DOIs
Publication statusPublished - 2021

Keywords

  • Automated systems
  • nosocomial infections
  • Pseudomonas aeruginosa
  • TDR-300B
  • VITEK®-MS
  • VITEK®2

Fingerprint

Dive into the research topics of 'Performance of TDR-300B and VITEK®2 for the identification of Pseudomonas aeruginosa in comparison with VITEK®-MS'. Together they form a unique fingerprint.

Cite this