Performance of ldbio aspergillus wb and ict antibody detection in chronic pulmonary aspergillosis

Anna Rozaliyani; Sresta Azahra; Asriyani Abdullah; Ayu Eka Fatril; Harmi Rosianawati; Erlina Burhan; Diah Handayani; Arief Riadi Arifin; Jamal Zaini; Mulyati Tugiran; Robiatul Adawiyah; Ridhawati Syam; Heri Wibowo; Retno Wahyuningsih; Chris Kosmidis; David W. Denning; FINDRA SETIANINGRUM

doi:10.3390/jof7040311

Performance of ldbio aspergillus wb and ict antibody detection in chronic pulmonary aspergillosis

Anna Rozaliyani, Sresta Azahra, Asriyani Abdullah, Ayu Eka Fatril, Harmi Rosianawati, Erlina Burhan, Diah Handayani, Arief Riadi Arifin, Jamal Zaini, Mulyati Tugiran, Robiatul Adawiyah, Ridhawati Syam, Heri Wibowo, Retno Wahyuningsih, Chris Kosmidis, David W. Denning, FINDRA SETIANINGRUM

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Abstract

The detection of Aspergillus antibody has a key role in the diagnosis of chronic pulmonary as-pergillosis. Western blot (WB) and immunochromatography (ICT) lateral flow detection of Aspergillus antibody can be used as confirmatory and screening assays but their comparative performance in TB patients is not known. This study investigated the performance of these assays among 88 post-tuberculosis patients with suspected CPA. Sensitivity, specificity, receiver operating curve (ROC), area under-curve (AUC) and the agreement between two assays were evaluated. Both WB and ICT showed good sensitivity (80% and 85%, respectively) for detection of Aspergillus antibodies. Substantial agreement (0.716) between these assays was also obtained. The highest AUC result (0.804) was achieved with the combination of WB and ICT. The global intensity of WB correlated with the severity of symptoms in CPA group (p = 0.001). The combination of WB and ICT may increase specificity in CPA diagnosis.

Original language	English
Article number	311
Journal	Journal of Fungi
Volume	7
Issue number	4
DOIs	https://doi.org/10.3390/jof7040311
Publication status	Published - Apr 2021

Keywords

Chronic pulmonary aspergillosis
Immunochromatography
Western blot

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/jof7040311

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Rozaliyani, A., Azahra, S., Abdullah, A., Fatril, A. E., Rosianawati, H., Burhan, E., Handayani, D., Arifin, A. R., Zaini, J., Tugiran, M., Adawiyah, R., Syam, R., Wibowo, H., Wahyuningsih, R., Kosmidis, C., Denning, D. W., & SETIANINGRUM, FINDRA. (2021). Performance of ldbio aspergillus wb and ict antibody detection in chronic pulmonary aspergillosis. Journal of Fungi, 7(4), Article 311. https://doi.org/10.3390/jof7040311

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title = "Performance of ldbio aspergillus wb and ict antibody detection in chronic pulmonary aspergillosis",

abstract = "The detection of Aspergillus antibody has a key role in the diagnosis of chronic pulmonary as-pergillosis. Western blot (WB) and immunochromatography (ICT) lateral flow detection of Aspergillus antibody can be used as confirmatory and screening assays but their comparative performance in TB patients is not known. This study investigated the performance of these assays among 88 post-tuberculosis patients with suspected CPA. Sensitivity, specificity, receiver operating curve (ROC), area under-curve (AUC) and the agreement between two assays were evaluated. Both WB and ICT showed good sensitivity (80% and 85%, respectively) for detection of Aspergillus antibodies. Substantial agreement (0.716) between these assays was also obtained. The highest AUC result (0.804) was achieved with the combination of WB and ICT. The global intensity of WB correlated with the severity of symptoms in CPA group (p = 0.001). The combination of WB and ICT may increase specificity in CPA diagnosis.",

keywords = "Chronic pulmonary aspergillosis, Immunochromatography, Western blot",

author = "Anna Rozaliyani and Sresta Azahra and Asriyani Abdullah and Fatril, {Ayu Eka} and Harmi Rosianawati and Erlina Burhan and Diah Handayani and Arifin, {Arief Riadi} and Jamal Zaini and Mulyati Tugiran and Robiatul Adawiyah and Ridhawati Syam and Heri Wibowo and Retno Wahyuningsih and Chris Kosmidis and Denning, {David W.} and FINDRA SETIANINGRUM",

note = "Funding Information: Funding: This research was partly funded by United Kingdom-Indonesia Joint Partnership on Infectious Diseases (Medical Research Council, Newton Fund, Ristekdikti) with a grant number NKB-282/UN2.RST/HKP.05.00/2020 and MR/S019898/1 and Universitas Indonesia through PUTI Grant with contract number NKB-1326/UN2.RST/HKP.05.00/2020. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = apr,

doi = "10.3390/jof7040311",

language = "English",

volume = "7",

journal = "Journal of Fungi",

issn = "2309-608X",

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Rozaliyani, A, Azahra, S, Abdullah, A, Fatril, AE, Rosianawati, H, Burhan, E, Handayani, D, Arifin, AR, Zaini, J, Tugiran, M, Adawiyah, R, Syam, R, Wibowo, H, Wahyuningsih, R, Kosmidis, C, Denning, DW & SETIANINGRUM, FINDRA 2021, 'Performance of ldbio aspergillus wb and ict antibody detection in chronic pulmonary aspergillosis', Journal of Fungi, vol. 7, no. 4, 311. https://doi.org/10.3390/jof7040311

TY - JOUR

T1 - Performance of ldbio aspergillus wb and ict antibody detection in chronic pulmonary aspergillosis

AU - Rozaliyani, Anna

AU - Azahra, Sresta

AU - Abdullah, Asriyani

AU - Fatril, Ayu Eka

AU - Rosianawati, Harmi

AU - Burhan, Erlina

AU - Handayani, Diah

AU - Arifin, Arief Riadi

AU - Zaini, Jamal

AU - Tugiran, Mulyati

AU - Adawiyah, Robiatul

AU - Syam, Ridhawati

AU - Wibowo, Heri

AU - Wahyuningsih, Retno

AU - Kosmidis, Chris

AU - Denning, David W.

AU - SETIANINGRUM, FINDRA

N1 - Funding Information: Funding: This research was partly funded by United Kingdom-Indonesia Joint Partnership on Infectious Diseases (Medical Research Council, Newton Fund, Ristekdikti) with a grant number NKB-282/UN2.RST/HKP.05.00/2020 and MR/S019898/1 and Universitas Indonesia through PUTI Grant with contract number NKB-1326/UN2.RST/HKP.05.00/2020. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/4

Y1 - 2021/4

N2 - The detection of Aspergillus antibody has a key role in the diagnosis of chronic pulmonary as-pergillosis. Western blot (WB) and immunochromatography (ICT) lateral flow detection of Aspergillus antibody can be used as confirmatory and screening assays but their comparative performance in TB patients is not known. This study investigated the performance of these assays among 88 post-tuberculosis patients with suspected CPA. Sensitivity, specificity, receiver operating curve (ROC), area under-curve (AUC) and the agreement between two assays were evaluated. Both WB and ICT showed good sensitivity (80% and 85%, respectively) for detection of Aspergillus antibodies. Substantial agreement (0.716) between these assays was also obtained. The highest AUC result (0.804) was achieved with the combination of WB and ICT. The global intensity of WB correlated with the severity of symptoms in CPA group (p = 0.001). The combination of WB and ICT may increase specificity in CPA diagnosis.

AB - The detection of Aspergillus antibody has a key role in the diagnosis of chronic pulmonary as-pergillosis. Western blot (WB) and immunochromatography (ICT) lateral flow detection of Aspergillus antibody can be used as confirmatory and screening assays but their comparative performance in TB patients is not known. This study investigated the performance of these assays among 88 post-tuberculosis patients with suspected CPA. Sensitivity, specificity, receiver operating curve (ROC), area under-curve (AUC) and the agreement between two assays were evaluated. Both WB and ICT showed good sensitivity (80% and 85%, respectively) for detection of Aspergillus antibodies. Substantial agreement (0.716) between these assays was also obtained. The highest AUC result (0.804) was achieved with the combination of WB and ICT. The global intensity of WB correlated with the severity of symptoms in CPA group (p = 0.001). The combination of WB and ICT may increase specificity in CPA diagnosis.

KW - Chronic pulmonary aspergillosis

KW - Immunochromatography

KW - Western blot

UR - http://www.scopus.com/inward/record.url?scp=85105015900&partnerID=8YFLogxK

U2 - 10.3390/jof7040311

DO - 10.3390/jof7040311

M3 - Article

AN - SCOPUS:85105015900

SN - 2309-608X

VL - 7

JO - Journal of Fungi

JF - Journal of Fungi

IS - 4

M1 - 311

ER -

Performance of ldbio aspergillus wb and ict antibody detection in chronic pulmonary aspergillosis

Abstract

Keywords

UN SDGs

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