PENINGKATAN AKTIVITAS LIPASE KAPANG LIMBAH KERNEL DAN NUT KELAPA SAWIT DENGAN RADIASI GAMA DAN ULTRAVIOLET

Aris Indriawan, Wibowo Mangunwardoyo, Dadang Suhendar, Trismilah Siswodarsono

Research output: Contribution to journalArticlepeer-review

Abstract

Molds isolated from oil palm waste sampled from Malingping, Lebak, Banten, West Java have the potential for lipase production. This study aimed to increase the fungal lipase activity with gamma radiation and ultraviolet light (UV). NA and KC mold spores were exposed to various gamma radiation doses of 1, 2, 3 and 4 kGy. The best of these NA and KC resulted mutants were followed by ultraviolet mutations for 1, 2, 3, and 4 hours, at dose of 0.1 J/cm2, 254 nm, 20 cm. Lipase activity was tested by the Lindfield method. The results showed that gamma radiation affected the lipase activity of NA1kGy mutants (8.58 U/mL) and KC1 kGy (8.25 U/mL), each increased the lipase activity by 4.6% and 3.13% from the wild type, respectively. Mutations with ultraviolet had an effect on mutant lipase activity of KC4H 10U/mL and NA3H 9.25 U/mL, each increased the lipase activity by 25% and 15.63% from the wild type, respectively. Based on phenotypic and phylogenetic (28srRNA) approaches, a mold of KC had a 100% similarity with Aspergillus fumigatus strain RA204.
Original languageIndonesian
Pages (from-to)188-195
JournalJurnal Bioteknologi & Biosains Indonesia (JBBI)
Volume5
Issue number2
DOIs
Publication statusPublished - 2018

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