This paper reviewed the ability of Polymerase Chain Reaction (PCR) in multiplying DNA in order to amplify an extremely small amount of sample using an in-vitro chemical reaction which is needed for various purposes such as diagnostic in dentistry. Polymerase chain reaction (PCR) is a technique of amplifying DNA in vitro which has been widely recognized as a powerful molecular-biology method. DNA template, primers, dNTP and DNA polymerase are needed, and this mixture will be exposed to three different temperatures for several cycles to multiply the DNA. The cycle consists of denaturation of double stranded DNA, annealing, and an extension phase which then will multiply the DNA twice. After n times of cycles, the amount of DNA will be multiplied 2n times.
|Journal of Dentistry Indonesia
|Published - 1998