@inproceedings{f459a957be6c4de5b30479e1d5f99834,
title = "Partial purification and evaluation of bromelain from pineapple stem (Ananas comosus) in cream based preparation and its in vitro anti-inflammatory activity",
abstract = "The aim of this research was to evaluate bromelain from pineapple stem in cream based preparation and to observe in vitro anti-inflammatory activity of the cream. Optimization of three different cream formulas were done to determine the most stable one. During four weeks of storage period, formula I with a mixture of Olivem 1000 and Tween 60 surfactants had the best physical form and emulsion stability compared to the other two formulas, with a pH value at 27°C of 5.40 ± 0.00 and 5.71 ± 0.008 at week 0 and week 4 respectively. The bromelain used as active substance in formula I cream preparation was isolated by partial purification of pineapple stem extract (Ananas comosus [L.] Merr) by fractionation method using ammonium sulfate followed by dialysis, with a specific activity of 155.58 U/mg. Evaluation results of bromelain activity in optimized cream preparation showed an enzyme activity of 0.52 U/mL. Penetration test for bromelain cream was done based on the Franz Diffusion Cell method. The results showed that in 8 hours, the cumulative penetrated amount of bromelain in cream preparation was 793.45 μg/cm2. The anti-inflammatory test using the HRBC method for cream preparation containing each commercial bromelain, isolated bromelain, and aspirin with the same concentration (2%), showed percent stability results of 34.41%, 32.06%, and 37.82% respectively. Thus, it could be concluded that cream preparations containing bromelain from pineapple stem have moderate anti-inflammatory activity.",
author = "K. Masbagusdanta and S. Setiasih and S. Handayani and S. Hudiyono",
note = "Publisher Copyright: {\textcopyright} 2020 Author(s).; 8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019 ; Conference date: 06-08-2019 Through 07-08-2019",
year = "2020",
month = jun,
day = "4",
doi = "10.1063/5.0001345",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Mohamad Rafi and Rudi Heryanto and Wahyuni, {Wulan Tri}",
booktitle = "8th International Conference of the Indonesian Chemical Society, ICICS 2019",
address = "United States",
}