Partial purification and evaluation of bromelain from pineapple stem (Ananas comosus) in cream based preparation and its in vitro anti-inflammatory activity

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Abstract

The aim of this research was to evaluate bromelain from pineapple stem in cream based preparation and to observe in vitro anti-inflammatory activity of the cream. Optimization of three different cream formulas were done to determine the most stable one. During four weeks of storage period, formula I with a mixture of Olivem 1000 and Tween 60 surfactants had the best physical form and emulsion stability compared to the other two formulas, with a pH value at 27°C of 5.40 ± 0.00 and 5.71 ± 0.008 at week 0 and week 4 respectively. The bromelain used as active substance in formula I cream preparation was isolated by partial purification of pineapple stem extract (Ananas comosus [L.] Merr) by fractionation method using ammonium sulfate followed by dialysis, with a specific activity of 155.58 U/mg. Evaluation results of bromelain activity in optimized cream preparation showed an enzyme activity of 0.52 U/mL. Penetration test for bromelain cream was done based on the Franz Diffusion Cell method. The results showed that in 8 hours, the cumulative penetrated amount of bromelain in cream preparation was 793.45 μg/cm2. The anti-inflammatory test using the HRBC method for cream preparation containing each commercial bromelain, isolated bromelain, and aspirin with the same concentration (2%), showed percent stability results of 34.41%, 32.06%, and 37.82% respectively. Thus, it could be concluded that cream preparations containing bromelain from pineapple stem have moderate anti-inflammatory activity.

Original languageEnglish
Title of host publication8th International Conference of the Indonesian Chemical Society, ICICS 2019
EditorsMohamad Rafi, Rudi Heryanto, Wulan Tri Wahyuni
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420021
DOIs
Publication statusPublished - 4 Jun 2020
Event8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019 - Bogor, Indonesia
Duration: 6 Aug 20197 Aug 2019

Publication series

NameAIP Conference Proceedings
Volume2243
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019
CountryIndonesia
CityBogor
Period6/08/197/08/19

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