TY - GEN
T1 - Partial purification and evaluation of bromelain from pineapple stem (Ananas comosus) in cream based preparation and its in vitro anti-inflammatory activity
AU - Masbagusdanta, K.
AU - Setiasih, S.
AU - Handayani, S.
AU - Hudiyono, S.
N1 - Publisher Copyright:
© 2020 Author(s).
PY - 2020/6/4
Y1 - 2020/6/4
N2 - The aim of this research was to evaluate bromelain from pineapple stem in cream based preparation and to observe in vitro anti-inflammatory activity of the cream. Optimization of three different cream formulas were done to determine the most stable one. During four weeks of storage period, formula I with a mixture of Olivem 1000 and Tween 60 surfactants had the best physical form and emulsion stability compared to the other two formulas, with a pH value at 27°C of 5.40 ± 0.00 and 5.71 ± 0.008 at week 0 and week 4 respectively. The bromelain used as active substance in formula I cream preparation was isolated by partial purification of pineapple stem extract (Ananas comosus [L.] Merr) by fractionation method using ammonium sulfate followed by dialysis, with a specific activity of 155.58 U/mg. Evaluation results of bromelain activity in optimized cream preparation showed an enzyme activity of 0.52 U/mL. Penetration test for bromelain cream was done based on the Franz Diffusion Cell method. The results showed that in 8 hours, the cumulative penetrated amount of bromelain in cream preparation was 793.45 μg/cm2. The anti-inflammatory test using the HRBC method for cream preparation containing each commercial bromelain, isolated bromelain, and aspirin with the same concentration (2%), showed percent stability results of 34.41%, 32.06%, and 37.82% respectively. Thus, it could be concluded that cream preparations containing bromelain from pineapple stem have moderate anti-inflammatory activity.
AB - The aim of this research was to evaluate bromelain from pineapple stem in cream based preparation and to observe in vitro anti-inflammatory activity of the cream. Optimization of three different cream formulas were done to determine the most stable one. During four weeks of storage period, formula I with a mixture of Olivem 1000 and Tween 60 surfactants had the best physical form and emulsion stability compared to the other two formulas, with a pH value at 27°C of 5.40 ± 0.00 and 5.71 ± 0.008 at week 0 and week 4 respectively. The bromelain used as active substance in formula I cream preparation was isolated by partial purification of pineapple stem extract (Ananas comosus [L.] Merr) by fractionation method using ammonium sulfate followed by dialysis, with a specific activity of 155.58 U/mg. Evaluation results of bromelain activity in optimized cream preparation showed an enzyme activity of 0.52 U/mL. Penetration test for bromelain cream was done based on the Franz Diffusion Cell method. The results showed that in 8 hours, the cumulative penetrated amount of bromelain in cream preparation was 793.45 μg/cm2. The anti-inflammatory test using the HRBC method for cream preparation containing each commercial bromelain, isolated bromelain, and aspirin with the same concentration (2%), showed percent stability results of 34.41%, 32.06%, and 37.82% respectively. Thus, it could be concluded that cream preparations containing bromelain from pineapple stem have moderate anti-inflammatory activity.
UR - http://www.scopus.com/inward/record.url?scp=85086663584&partnerID=8YFLogxK
U2 - 10.1063/5.0001345
DO - 10.1063/5.0001345
M3 - Conference contribution
AN - SCOPUS:85086663584
T3 - AIP Conference Proceedings
BT - 8th International Conference of the Indonesian Chemical Society, ICICS 2019
A2 - Rafi, Mohamad
A2 - Heryanto, Rudi
A2 - Wahyuni, Wulan Tri
PB - American Institute of Physics Inc.
T2 - 8th International Conference of the Indonesian Chemical Society 2019, ICICS 2019
Y2 - 6 August 2019 through 7 August 2019
ER -