Paenibacillus cisolokensis sp. nov., isolated from litter of a geyser

Akira Yokota, Fitria Ningsih, Dafina Ghossani Nurlaili, Yasuteru Sakai, Shuhei Yabe, Ariyanti Oetari, Iman Santoso, Wellyzar Sjamsuridzal

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A Gram-stain-positive, endospore-forming, aerobic and thermophilic bacterium, designated strain LC2-13AT, was isolated from Cisolok geyser, West Java, Indonesia, at 50 °C. The isolate was rod-shaped and motile by means of peritrichous flagella. The major cellular fatty acids were iso-C16:0, C16:0 and anteiso-C15:0 and the major quinone was menaquinone 7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content was 56.6 mol% and the major diagnostic diamino acid in the cellwall peptidoglycan was meso-diaminopimelic acid. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain LC2-13AT is related most closely to Paenibacillus kobensis DSM 10249T (94.86% similarity), Paenibacillus tarimensis SA-7-6T (94.77 %) and Paenibacillus barengoltzii SAFN-016T (94.77 %). On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain LC2-13AT is affiliated to the genus Paenibacillus, but could be distinguished from recognized species of this genus. A novel species with the name Paenibacillus cisolokensis sp. nov. is thus proposed. The type strain is LC2-13AT (=UICC B-42T=NRRL B-65368T=DSM 101873T).

Original languageEnglish
Article number001151
Pages (from-to)3088-3094
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number8
DOIs
Publication statusPublished - 1 Aug 2016

Keywords

  • Cisolok geyser
  • Paenibacillus cisolokensis sp. nov.
  • Thermophilic bacterium

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