Paclitaxel is evidence to reduce growing ovarian follicle growth in mice model study

Mila Maidarti, Wataru Tarumi, Seido Takae, Budi Wiweko, Nao Suzuki

Research output: Contribution to journalArticlepeer-review

Abstract

We aimed to investigate the effects of paclitaxel on ovarian follicle development and oocyte meiotic competence. Early secondary follicles were cultured individually with or without paclitaxel 2.5 × 10−10, 2.5 × 10−9, and 2.5 × 10−8 M for 12 days. Thereafter, the follicles were treated with human chorionic gonadotropin (hCG) and epidermal growth factor (EGF). Follicle morphology, oocytes meiotic maturation, immunofluorescence for α-tubulin of the oocytes, mRNA expression of growth differentiation factor 9 (GDF9), B-cell lymphoma 2 (Bcl2), and Bcl2-associated X (Bax) were examined every 4, 8, and 12 days of the culture period. We demonstrated that high dose paclitaxel treatment decreased follicle survival (p ≤ 0.05), while lower dose (2.5 × 10−9 M) reduced the survival compared to control after 12 days of culture. The number of oocytes at MII stage was not significantly different between control and paclitaxel groups (p > 0.05). Paclitaxel increased GDF9 expression in oocytes after 4 days of the culture (p ≤ 0.05). Bcl2 declined significantly compared to control after 8 days (p ≤ 0.05 for all groups), while Bax expression tended to be consistent (p ≥ 0.05 for all groups). To conclude, high concentration paclitaxel reduces follicle preantral follicle growth, while in lower concentration it decreases more growing follicle growth and survival.

Original languageEnglish
Article number105386
JournalToxicology in Vitro
Volume83
DOIs
Publication statusPublished - Sep 2022

Keywords

  • Follicle culture
  • Follicle development
  • Follicle survival
  • Oocytes meiotic competence
  • Paclitaxel

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