Oxidative stress tolerance of early stage diabetic endothelial progenitor cell

Dewi Sukmawati, Satoshi Fujimura, Sachie Jitsukawa, Rie Ito-Hirano, Takamasa Ishii, Tadayuki Sato, Ayato Hayashi, Seigo Itoh, Hiroshi Mizuno, Hiroyuki Daida, Rica Tanaka

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Introduction: One of the causes for poor vasculogenesis of diabetes mellitus (DM) is known to rise from the dysfunction of bone marrow-derived endothelial progenitor cells (BM EPCs). However, the origin of its cause is less understood. We aimed to investigate the effect of oxidative stress in early stage of diabetic BM-EPC and whether its vasculogenic dysfunction is caused by oxidative stress. Methods: Bone marrow c-Kit+Sca-1+Lin- (BM-KSL) cells were sorted from control and streptozotocin-induced diabetic C57BL6J mice by flow cytometry. BM-KSLs were then assessed for vasculogenic potential (colony forming assay; EPC-CFA), accumulation of intracellular ROS (CM-H2DCFDA), carbonylated protein (ELISA), anti-oxidative enzymes expression (RT-qPCR) and catalase activity (Amplex Red). Results: Compared to control, DM BM-KSL had significantly lower EPC-CFUs in both definitive EPC-CFU and total EPC-CFU (p<0.05). Interestingly, the oxidative stress level of DM BM-KSL was comparable and was not significantly different to control followed by increased in anti-oxidative enzymes expression and catalase activity. Conclusions: Primitive BM-EPCs showed vasculogenic dysfunction in early diabetes. However the oxidative stress is not denoted as the major initiating factor of its cause. Our results suggest that primitive BM-KSL cell has the ability to compensate oxidative stress levels in early diabetes by increasing the expression of anti-oxidative enzymes.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalRegenerative Therapy
Volume1
DOIs
Publication statusPublished - 1 Jun 2015

Keywords

  • Anti-oxidative enzymes
  • BM-EPC
  • BM-KSL
  • Bone marrow-derived endothelial progenitor cells
  • DEPC-CFU
  • Diabetes
  • DM BM-KSL
  • EPC-CFA
  • EPC-CFU
  • Oxidative stress
  • PEPC-CFU
  • QQc
  • TEPC-CFU

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