@inproceedings{de47d75c798d45419c2162f01fbe60f9,
title = "Oxidation of extracted cholesterol from fatty food by using crude cholesterol oxidase Streptomyces sp.",
abstract = "Cholesterol is contained in many food ingredients from animal origin. One of methods that is used to degrade cholesterol is an enzymatic oxidation. Numerous researcher have been done in cholesterol enzymatic oxidation by used commercial cholesterol as a substrate. In this study, the substrate is obtained by extracting crude cholesterol from fatty food. The aim of this study is to oxidize the crude extract cholesterol from egg yolk, chicken liver, chicken meat, and beef by using cholesterol oxidase enzyme extracted from Streptomyces sp. Oxidation test was carried out on certain constant parameters by quantifying the results using HPLC. The cholesterol had been extracted by mixing the fatty foods with non- polar solvent. The sources for crude extract cholesterol such as egg yolk, chicken liver, and chicken meat were degraded until 20% with 2 mg/mL cholesterol oxidase after 3 hours reaction, meanwhile the crude extract cholesterol from beef was able to oxidize 10% of cholesterol.",
keywords = "cholesterol, cholesterol oxidase, enzymatic reaction, fatty foods, oxidation",
author = "Perdani, {Meka Saima} and Muhammad Faturrohman and Putri, {Dwini Normayulisa} and Heri Hermansyah",
year = "2019",
month = dec,
day = "10",
doi = "10.1063/1.5139357",
language = "English",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Kenny Lischer and Tomy Abuzairi and Rahman, {Siti Fauziyah} and Misri Gozan",
booktitle = "4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices",
note = "4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 ; Conference date: 22-07-2019 Through 24-07-2019",
}