Oxidation of extracted cholesterol from fatty food by using crude cholesterol oxidase Streptomyces sp.

Meka Saima Perdani, Muhammad Faturrohman, Dwini Normayulisa Putri, Heri Hermansyah

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Cholesterol is contained in many food ingredients from animal origin. One of methods that is used to degrade cholesterol is an enzymatic oxidation. Numerous researcher have been done in cholesterol enzymatic oxidation by used commercial cholesterol as a substrate. In this study, the substrate is obtained by extracting crude cholesterol from fatty food. The aim of this study is to oxidize the crude extract cholesterol from egg yolk, chicken liver, chicken meat, and beef by using cholesterol oxidase enzyme extracted from Streptomyces sp. Oxidation test was carried out on certain constant parameters by quantifying the results using HPLC. The cholesterol had been extracted by mixing the fatty foods with non- polar solvent. The sources for crude extract cholesterol such as egg yolk, chicken liver, and chicken meat were degraded until 20% with 2 mg/mL cholesterol oxidase after 3 hours reaction, meanwhile the crude extract cholesterol from beef was able to oxidize 10% of cholesterol.

Original languageEnglish
Title of host publication4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2019
EditorsKenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419445
DOIs
Publication statusPublished - 10 Dec 2019
Event4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia
Duration: 22 Jul 201924 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2193
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
CountryIndonesia
CityPadang, West Sumatera
Period22/07/1924/07/19

Keywords

  • cholesterol
  • cholesterol oxidase
  • enzymatic reaction
  • fatty foods
  • oxidation

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