Optimum concentration of platelet-rich fibrin lysate for human dental pulp stem cells culture medium

Sandy Ratna Asri, Hasti Dwi Setiati, Dini Asrianti, Anggraini Margono, Munyati Usman, Indah Yulianto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Platelet Rich Fibrin Lysate (L-PRF) contains platelets, leukocytes and growth factors, all of which contribute to proliferation and healing processes. This allogeneic and autologous material can be used for cell culture supplementation. Human dental pulp stem cells (hDPSCs) were isolated from the impacted third molars of 10 healthy donors, and then cultured in 3 different supplemented media cultures (10%, 20% and 25% L-PRF). Cell proliferation was analyzed using flow citometry and MTT-Assay. Compared to cells without supplementation (control group), the L-PRF group showed significant hDPSC proliferation on day 1 (p < 0.05), with the highest proliferation rate observed in the 25% L-PRF group. Significant proliferation was also observed between day 1 and 3 and between day 1 and 5 (p < 0.05) in the groups with 10%, 20% and 25% L-PRF supplementation. There is no significant proliferation observed between day 1, 3 and 5 in the control group (p > 0.05). The 10%, 20% and 25% L-PRF groups can serve as supplemental culture media for hDPSCs proliferation, with the highest proliferative potential observed in the 25% L-PRF group.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalJournal of International Dental and Medical Research
Volume12
Issue number1
Publication statusPublished - 1 Jan 2019

Keywords

  • Human dental pulp stem cells
  • Platelet-rich fibrin lysate
  • Proliferation

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