A modified glassy carbon electrode (GCE) was developed based on a synthesized graphene oxide (GO) gold nanorod (AuNR) decorated composite (GO/AuNR) for sensitive electrochemical sensing of uric acid (UA). The electrochemical performance of GO/AuNR/GCE for UA detection was investigated employing the differential pulse voltammetry (DPV) technique. Central composite design (CCD) was applied to obtain the optimum composition of the GO and AuNR composite, which provide the highest possible UA oxidation peak current. The optimum composition was obtained at a GO concentration of 5 mg mL−1 and AuNR volume of 10 mL. Under the optimum conditions, GO/AuNR/GCE showed acceptable analytical performance for UA detection with good linearity (concentration range of 10-90 μM) and both a low detection limit (0.4 μM) and quantitation limit (1.0 μM). Furthermore, the proposed sensor exhibits superior stability, reproducibility, and selectivity using ascorbic acid (AA), dopamine (DA), urea, glucose, and magnesium as interferents. Finally, practical use of GO/AuNR/GCE was demonstrated by successfully determining the content of UA in human urine samples with the standard addition approach.