TY - JOUR
T1 - Optimization of ultrasonic extraction parameters for enhanced total flavonoid content from Jengkol peel
AU - Emilia, Amanda
AU - Hasna, Rafifah
AU - Arbianti, Rita
AU - Utami, Tania Surya
N1 - Publisher Copyright:
© 2024 Author(s).
PY - 2024/11/25
Y1 - 2024/11/25
N2 - Jengkol, a commonly consumed food ingredient, is frequently utilized without its outer layer. However, the outer peel of the jengkol fruit harbors a multitude of secondary metabolites that hold potential as bioinsecticides. Among these compounds, flavonoids stand out as toxic to insects. The quantification of flavonoid content within jengkol peel extracts is denoted as TFC (Total Flavonoid Content). Employing ultrasonic wave extraction, the conversion of jengkol peel into bioinsecticides can be facilitated. This research employs ultrasonic wave extraction, utilizing an ethanol solvent, with a frequency of 53 kHz and a temperature of 40℃. Through systematic variation of sonication duration and solvent concentration, this investigation scrutinizes the impact of these variables on TFC values and extracts yields. The assessment of TFC is conducted using UV-Vis spectrophotometry and referencing a quercetin standard solution. Optimal TFC output, specifically 1.643±0.026 mg QE/g of dried jengkol peel extract, is achieved at a 60-minute extraction period using 70% ethanol solvent. Liquid Chromatography-Mass Spectrometry (LCMS) analysis identifies various compounds with bioinsecticidal potential, including phenolic acids, fatty acids, flavonoids, phytoalexins, and coumarins, exhibiting the highest TFC levels. Employing ANOVA analysis followed by a post hoc LSD (Least Significance Different) test, it is evident with 95% confidence that both extraction time and solvent concentration significantly influence flavonoid content within jengkol peel extracts.
AB - Jengkol, a commonly consumed food ingredient, is frequently utilized without its outer layer. However, the outer peel of the jengkol fruit harbors a multitude of secondary metabolites that hold potential as bioinsecticides. Among these compounds, flavonoids stand out as toxic to insects. The quantification of flavonoid content within jengkol peel extracts is denoted as TFC (Total Flavonoid Content). Employing ultrasonic wave extraction, the conversion of jengkol peel into bioinsecticides can be facilitated. This research employs ultrasonic wave extraction, utilizing an ethanol solvent, with a frequency of 53 kHz and a temperature of 40℃. Through systematic variation of sonication duration and solvent concentration, this investigation scrutinizes the impact of these variables on TFC values and extracts yields. The assessment of TFC is conducted using UV-Vis spectrophotometry and referencing a quercetin standard solution. Optimal TFC output, specifically 1.643±0.026 mg QE/g of dried jengkol peel extract, is achieved at a 60-minute extraction period using 70% ethanol solvent. Liquid Chromatography-Mass Spectrometry (LCMS) analysis identifies various compounds with bioinsecticidal potential, including phenolic acids, fatty acids, flavonoids, phytoalexins, and coumarins, exhibiting the highest TFC levels. Employing ANOVA analysis followed by a post hoc LSD (Least Significance Different) test, it is evident with 95% confidence that both extraction time and solvent concentration significantly influence flavonoid content within jengkol peel extracts.
UR - http://www.scopus.com/inward/record.url?scp=85212194576&partnerID=8YFLogxK
U2 - 10.1063/5.0236769
DO - 10.1063/5.0236769
M3 - Conference article
AN - SCOPUS:85212194576
SN - 0094-243X
VL - 3215
JO - AIP Conference Proceedings
JF - AIP Conference Proceedings
IS - 1
M1 - 070002
T2 - 18th International Conference on Quality in Research, QiR 2023
Y2 - 23 October 2023 through 25 October 2023
ER -