Optimization of ultrasonic extraction parameters for enhanced total flavonoid content from Jengkol peel

Amanda Emilia, Rafifah Hasna, Rita Arbianti, Tania Surya Utami

Research output: Contribution to journalConference articlepeer-review

Abstract

Jengkol, a commonly consumed food ingredient, is frequently utilized without its outer layer. However, the outer peel of the jengkol fruit harbors a multitude of secondary metabolites that hold potential as bioinsecticides. Among these compounds, flavonoids stand out as toxic to insects. The quantification of flavonoid content within jengkol peel extracts is denoted as TFC (Total Flavonoid Content). Employing ultrasonic wave extraction, the conversion of jengkol peel into bioinsecticides can be facilitated. This research employs ultrasonic wave extraction, utilizing an ethanol solvent, with a frequency of 53 kHz and a temperature of 40℃. Through systematic variation of sonication duration and solvent concentration, this investigation scrutinizes the impact of these variables on TFC values and extracts yields. The assessment of TFC is conducted using UV-Vis spectrophotometry and referencing a quercetin standard solution. Optimal TFC output, specifically 1.643±0.026 mg QE/g of dried jengkol peel extract, is achieved at a 60-minute extraction period using 70% ethanol solvent. Liquid Chromatography-Mass Spectrometry (LCMS) analysis identifies various compounds with bioinsecticidal potential, including phenolic acids, fatty acids, flavonoids, phytoalexins, and coumarins, exhibiting the highest TFC levels. Employing ANOVA analysis followed by a post hoc LSD (Least Significance Different) test, it is evident with 95% confidence that both extraction time and solvent concentration significantly influence flavonoid content within jengkol peel extracts.

Original languageEnglish
Article number070002
JournalAIP Conference Proceedings
Volume3215
Issue number1
DOIs
Publication statusPublished - 25 Nov 2024
Event18th International Conference on Quality in Research, QiR 2023 - Bali, Indonesia
Duration: 23 Oct 202325 Oct 2023

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