Effects of sonication and hydrothermal treatment time on TiO2 nanowires (TNWs) formation have been investigated. Sonication of TiO2 P25 sol was performed using sonicator water bath Cole for 0.5, 1 and 2 hours followed by hydrothermal treatment. The hydrothermal treatment was carried out in a teflon lined stainless steel autoclave for 12, 15 and 24 hours. The samples were characterized by means of Scanning Electron Microscope (SEM), X-ray powder diffractometer (XRD), UV-vis diffuse and reflectance spectroscopy (UV-vis DRS). The specific surface area of each sample was determined by the BET nitrogen gas adsorption/desorption method. The photocatalytic activity of TNWs was evaluated with photocatalytic H2 evolution from aqueous methanol solution. The result showed that the best TNWs was obtained at 1 hr sonication followed by hydrothermal process for 12 hours. By using this catalyst hydrogen production reached 82 μmol after 5h while using TiO2 P25 could only reach 23 μmol.