Optimization of Solanesol Extraction from Nicotiana tabacum L Virginia Leaves

Felicia Carissa, Edwin Rizki Safitra, Alvian Nuriansyah, Isnandito Duarsa, Misri Gozan

Research output: Contribution to journalConference articlepeer-review

Abstract

Solanesol is mainly used as an intermediate compound to form Q10 co-enzyme and vitamin K2. Several extraction methods such as Microwave-Assisted Extraction (MAE) have been developed to extract solanesol. However, existing MAE methods require long extraction times and complex equipment with low yield. This study objective is to obtain the most efficient solanesol extraction method. Different ratios of Hexane: Ethanol or Petroleum Ether: Ethanol as organic solvents were tested. Tobacco leaves collected from East Java were oven-dried and ground until 80 mesh. The extract obtained was further purified through rotary and column chromatography. Silica gel was used in the column as the stationary phase and PE: Ethanol as the mobile phase. The results show that the optimum MAE method to extract solanesol from tobacco leaves is using the ratio of tobacco weight per volume of water was 0.25 gr/ml, microwave power at 200 Watts, and extraction time of 1.5 minutes. The optimum maceration condition to extract solanesol from tobacco leaves was using PE: Ethanol with the ratio of 3:2 for 4 hours by using a column with a D/L ratio of 1:5. Analysis with HPLC shows the highest solanesol obtained from the optimum condition was 1.3% (w/w) with microwave operating conditions at 1.5 minutes and 200 watts. This operating condition varies from the existing research. It also held a longer microwave duration with lower power usage. The process of MAE helps lysis molecular walls, with additional NaOH helping release solanesol from other compounds inside the tobacco. Solanesol is then extracted with non-polar solvents with the same solubility as solanesol. The extract then goes through a separation process by column chromatography which separates solanesol based on differences in polarity, and capabilities of the stationary and mobile phases. Previous studies show that the maximum solanesol content can be obtained is at 0.91% (w/w) while this work obtains more solanesol with less time and simpler equipment.

Original languageEnglish
Article number070002
JournalAIP Conference Proceedings
Volume3080
Issue number1
DOIs
Publication statusPublished - 7 Mar 2024
Event15th Asian Congress on Biotechnology in conjunction with the 7th International Symposium on Biomedical Engineering, ACB-ISBE 2022 - Bali, Indonesia
Duration: 2 Oct 20226 Oct 2022

Keywords

  • Column Chromatography
  • Maceration
  • Microwave Assisted Extraction
  • Solanesol
  • Tobacco

Fingerprint

Dive into the research topics of 'Optimization of Solanesol Extraction from Nicotiana tabacum L Virginia Leaves'. Together they form a unique fingerprint.

Cite this