Optimization of sodium deoxycholate-based transfersomes for percutaneous delivery of peptides and proteins

Astried Leonyza, Silvia Surini

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Objective: This study aims to formulate and characterize the transfersomes for percutaneous delivery of peptides and proteins. In particular, this study was a preliminary study for the transfersomes formulation of recombinant human epidermal growth factor (rhEGF) for topical delivery. Methods: The transfersomes was prepared by thin film hydration method using phosphatidylcholine and sodium deoxycholate as vesicle former. In this study, transfersomes formulas were optimized, namely TF1, TF2, TF3, and TF4 with several ratios of phospholipid and surfactant which were 90:10, 85:15, 80:20, and 75:25, respectively. Afterward, the transfersomes were characterized in terms of particle size distribution, polydispersity index, zeta potential, morphology of vesicles, and deformability index. Results: The results showed that the best formulation was TF3 with the ratio of 80:20 with a particle size of 118.6±1.33 nm, polydispersity index of 0.102±0.011, zeta potential of-30.9±0,46 mV, and deformability index of 1.182±0.08. TEM analysis also showed spherical and unilamellar vesicles of transfersomes. Conclusion: This work demonstrated that the sodium deoxycholate-based transfersomes could be potential to be further formulated with peptide and protein for percutaneous delivery.

Original languageEnglish
Pages (from-to)329-332
Number of pages4
JournalInternational Journal of Applied Pharmaceutics
Volume11
Issue number5
DOIs
Publication statusPublished - 1 Sep 2019

Keywords

  • Peptides
  • Percutaneous delivery
  • Proteins
  • Recombinant human epidermal growth factor
  • Sodium deoxycholate
  • Transfersomes

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