Optimization of preparation steps for Majalaya carp (Cyprinus carpio Linn.) chromosome study from gills

S. W.R. Hilia, S. H.M. Aundi, W. Wardhana, A. Dwiranti

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Majalaya Carp (Cyprinus carpio L.) is one of the most important aquaculture species in Indonesia. This freshwater fish has a great opportunity to be developed, thus, the cultivation data, starting from data on hatchery, nursery, enlargement, to harvest, are available. However, cytogenetic studies of Majalaya carp has been limited. One of the challenges for the chromosomal study is preparing a well-spread chromosome. The objective of this research was to optimize the preparation method to study the chromosome of Majalaya carp from gills. The optimized steps included synchronization (1, 2, 3 h of colcemid treatment); hydrolysis (8, 10, 15 min of KCl treatment); and fixation (1, 2, 3 times). The results showed that the optimum colcemid treatment to synchronize cells at metaphase, KCl incubation for hydrolysis, fixation, and staining method were 2 h, 8 min, and once fixative respectively. By using these optimum conditions obtained from this study, chromosomes of Majalaya carp could successfully be obtained. The results of this study could give insight into further chromosomal analysis.

Original languageEnglish
Title of host publicationProceedings of the 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
EditorsTerry Mart, Djoko Triyono, Tribidasari Anggraningrum Ivandini
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735420014
DOIs
Publication statusPublished - 1 Jun 2020
Event5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019 - Depok, Indonesia
Duration: 9 Jul 201910 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2242
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
CountryIndonesia
CityDepok
Period9/07/1910/07/19

Keywords

  • Chromosome
  • Cyprinus carpio
  • gills
  • Majalaya carp
  • optimization

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