TY - GEN
T1 - Optimization of preparation steps for Majalaya carp (Cyprinus carpio Linn.) chromosome study from gills
AU - Hilia, S. W.R.
AU - Aundi, S. H.M.
AU - Wardhana, W.
AU - Dwiranti, A.
N1 - Publisher Copyright:
© 2020 Author(s).
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Majalaya Carp (Cyprinus carpio L.) is one of the most important aquaculture species in Indonesia. This freshwater fish has a great opportunity to be developed, thus, the cultivation data, starting from data on hatchery, nursery, enlargement, to harvest, are available. However, cytogenetic studies of Majalaya carp has been limited. One of the challenges for the chromosomal study is preparing a well-spread chromosome. The objective of this research was to optimize the preparation method to study the chromosome of Majalaya carp from gills. The optimized steps included synchronization (1, 2, 3 h of colcemid treatment); hydrolysis (8, 10, 15 min of KCl treatment); and fixation (1, 2, 3 times). The results showed that the optimum colcemid treatment to synchronize cells at metaphase, KCl incubation for hydrolysis, fixation, and staining method were 2 h, 8 min, and once fixative respectively. By using these optimum conditions obtained from this study, chromosomes of Majalaya carp could successfully be obtained. The results of this study could give insight into further chromosomal analysis.
AB - Majalaya Carp (Cyprinus carpio L.) is one of the most important aquaculture species in Indonesia. This freshwater fish has a great opportunity to be developed, thus, the cultivation data, starting from data on hatchery, nursery, enlargement, to harvest, are available. However, cytogenetic studies of Majalaya carp has been limited. One of the challenges for the chromosomal study is preparing a well-spread chromosome. The objective of this research was to optimize the preparation method to study the chromosome of Majalaya carp from gills. The optimized steps included synchronization (1, 2, 3 h of colcemid treatment); hydrolysis (8, 10, 15 min of KCl treatment); and fixation (1, 2, 3 times). The results showed that the optimum colcemid treatment to synchronize cells at metaphase, KCl incubation for hydrolysis, fixation, and staining method were 2 h, 8 min, and once fixative respectively. By using these optimum conditions obtained from this study, chromosomes of Majalaya carp could successfully be obtained. The results of this study could give insight into further chromosomal analysis.
KW - Chromosome
KW - Cyprinus carpio
KW - gills
KW - Majalaya carp
KW - optimization
UR - http://www.scopus.com/inward/record.url?scp=85086660976&partnerID=8YFLogxK
U2 - 10.1063/5.0008276
DO - 10.1063/5.0008276
M3 - Conference contribution
AN - SCOPUS:85086660976
T3 - AIP Conference Proceedings
BT - Proceedings of the 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
A2 - Mart, Terry
A2 - Triyono, Djoko
A2 - Ivandini, Tribidasari Anggraningrum
PB - American Institute of Physics Inc.
T2 - 5th International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2019
Y2 - 9 July 2019 through 10 July 2019
ER -