Optimization of power, duration, and water in solanesol extraction from virginia nicotiana tabacum l. leaves assisted by microwaveultrasound using response surface methodology

Solanesol is an aliphatic terpene alcohol composed of nine isoprene units with many bioactive benefits and is commonly found in Solanaceae, including tobacco. Solanesol is difficult to synthesize artificially; Therefore, acquisition still relies on extraction. Extraction methods such as microwave-assisted extraction (MAE) and ultrasoundassisted extraction (UAE) have reduced extraction time and solvent consumption. Some variables that influence extraction performance in MAE are solvent selection, extraction time, microwave power, and sample-tosolvent ratio (S/F). This research aims to find the optimum parameters for the extraction of solanesol from Virginia variety tobacco leaves using the response surface methodology approach. This study considered the extraction time (30, 60, and 90 seconds), sample-to-water ratio (S/F) (1:1, 1:3, and 1:6), and microwave power (200, 400, and 600 W) were optimized. Solanesol from the extraction was quantified using high-performance liquid chromatography (HPLC), and the yield response was then processed using Design Expert to obtain optimum variables of MAE. This study shows that the optimum condition for the MAE process was 10 mL of water, with a power of 324.581 W for 30 seconds and a predicted solanesol yield of 2.926%.