TY - JOUR
T1 - Optimization of laccase production from a newly isolated Trametes sp. EDN134
AU - Ningsih, F.
AU - Yanto, D. H.Y.
AU - Mangunwardoyo, W.
AU - Anita, S. H.
AU - Watanabe, T.
N1 - Funding Information:
This work was partly supported by LIPI through DIPA of Research Center for Biology 2018 under Indonesian Biodiversity Strategy and Action Plan (IBSAP) program and Japan ASEAN Science, Technology, and Innovation Platform (JASTIP) Program, RISH Kyoto University, Japan.
Publisher Copyright:
© the author(s)
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10/7
Y1 - 2020/10/7
N2 - Laccase is one important enzyme in decolorization of textile dyes. This research aims to study the optimization of laccase production from Trametes sp. EDN134 under several variation of substrates (bagasse, bamboo, Jabon wood, oil palm empty fruit bunch (OPEFB), and sorghum fibers), time incubation (1-15 d), growth temperature (25, 27, 30, 32, 35 and 37oC), and concentrations of inducer CuSO4 (1, 2, 3, 4 and 5 mM). The results showed that optimum laccase specific activity (14,838 U/g) detected in the OPEFB medium at 10 days incubation, temperature 30oC and CuSO4 2 mM. The study suggests that these parameters could be used for the optimization of laccase production from a newly isolated Trametes sp. EDN134.
AB - Laccase is one important enzyme in decolorization of textile dyes. This research aims to study the optimization of laccase production from Trametes sp. EDN134 under several variation of substrates (bagasse, bamboo, Jabon wood, oil palm empty fruit bunch (OPEFB), and sorghum fibers), time incubation (1-15 d), growth temperature (25, 27, 30, 32, 35 and 37oC), and concentrations of inducer CuSO4 (1, 2, 3, 4 and 5 mM). The results showed that optimum laccase specific activity (14,838 U/g) detected in the OPEFB medium at 10 days incubation, temperature 30oC and CuSO4 2 mM. The study suggests that these parameters could be used for the optimization of laccase production from a newly isolated Trametes sp. EDN134.
UR - http://www.scopus.com/inward/record.url?scp=85092654489&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/572/1/012024
DO - 10.1088/1755-1315/572/1/012024
M3 - Conference article
AN - SCOPUS:85092654489
SN - 1755-1307
VL - 572
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012024
T2 - 9th International Symposium for Sustainable Humanosphere, ISSH 2019
Y2 - 28 October 2019 through 29 October 2019
ER -