TY - GEN
T1 - Oil immersion technique for cellular microencapsulation
AU - Nurhayati, Retno Wahyu
AU - Mubarok, Wildan
AU - Cahyo, Rafianto Dwi
AU - Alawiyah, Kamila
N1 - Funding Information:
This work was supported by Q1Q2 Scheme Research Grant from Universitas Indonesia (Hibah Publikasi Artikel di Jurnal Internasional Kuartil Q1 dan Q2 (Q1Q2) No. NKB-0232/UN2.R3.1/HKP.05.00/2019) awarded to Dr. Retno Wahyu Nurhayati. The authors thank Prof. Jeanne Adiwinata Pawitan for the valuable discussion and Mr. Dimas Ramadhian Noor from Human Cancer Research Center, IMERI, Faculty of Medicine, Universitas Indonesia for Fluorescence Microscope Analysis.
Publisher Copyright:
© 2019 Author(s).
PY - 2019/12/10
Y1 - 2019/12/10
N2 - Recent advances in tissue engineering application for medical intervention has created a need for three-dimensional (3D) cellular microenvironment in the form of cellular microencapsulation. Cellular microencapsulation can be used in several applications such as in vitro drug screening as well as cellular transplantation. It is important to note, however, that despite all the benefit from the cells, the capsule itself also has a major role. Significant impact and even clinical risks can present if the capsule is not biocompatible or even toxic. Hence, careful consideration on the materials and more importantly the fabrication process of the capsule is needed. In this work, we presented a method for cellular encapsulation using oil immersion technique. Briefly, suspension of K562 cells and collagen was injected into the mineral oil on a cell strainer. Microcapsule then washed with PBS and immersed with culture medium. Photomicrograph analysis was performed to evaluate the shape as well as to measure the volume of the microcapsule. Immunohistochemistry staining with Propidium Iodide (PI) was used to confirm K562 entrapment within the microcapsule. Cellular microencapsulation using oil immersion technique resulted in a rounded sphere. The increased volume of cells-hydrogel suspension resulted in bigger diameter and volume of the capsule. Due to the nature of the immersion process, a layer of oil could be found outside the capsule. The volume of the oil on the border of the capsule also increased with higher hydrogel-cells suspension. PI-stained K562 cells were found within the microcapsule, confirming that K562 cells were indeed entrapped within the microcapsule. In conclusion, oil immersion technique provides a quick, easy, and feasible method to produce cellular microencapsulation.
AB - Recent advances in tissue engineering application for medical intervention has created a need for three-dimensional (3D) cellular microenvironment in the form of cellular microencapsulation. Cellular microencapsulation can be used in several applications such as in vitro drug screening as well as cellular transplantation. It is important to note, however, that despite all the benefit from the cells, the capsule itself also has a major role. Significant impact and even clinical risks can present if the capsule is not biocompatible or even toxic. Hence, careful consideration on the materials and more importantly the fabrication process of the capsule is needed. In this work, we presented a method for cellular encapsulation using oil immersion technique. Briefly, suspension of K562 cells and collagen was injected into the mineral oil on a cell strainer. Microcapsule then washed with PBS and immersed with culture medium. Photomicrograph analysis was performed to evaluate the shape as well as to measure the volume of the microcapsule. Immunohistochemistry staining with Propidium Iodide (PI) was used to confirm K562 entrapment within the microcapsule. Cellular microencapsulation using oil immersion technique resulted in a rounded sphere. The increased volume of cells-hydrogel suspension resulted in bigger diameter and volume of the capsule. Due to the nature of the immersion process, a layer of oil could be found outside the capsule. The volume of the oil on the border of the capsule also increased with higher hydrogel-cells suspension. PI-stained K562 cells were found within the microcapsule, confirming that K562 cells were indeed entrapped within the microcapsule. In conclusion, oil immersion technique provides a quick, easy, and feasible method to produce cellular microencapsulation.
KW - Immersion
KW - K562
KW - Microencapsulation
KW - Oil
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=85076781922&partnerID=8YFLogxK
U2 - 10.1063/1.5139325
DO - 10.1063/1.5139325
M3 - Conference contribution
AN - SCOPUS:85076781922
T3 - AIP Conference Proceedings
BT - 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
A2 - Lischer, Kenny
A2 - Abuzairi, Tomy
A2 - Rahman, Siti Fauziyah
A2 - Gozan, Misri
PB - American Institute of Physics Inc.
T2 - 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Y2 - 22 July 2019 through 24 July 2019
ER -