Non-fecal and fecal coliform tests of ready-to-eat food and drinks using fluorogenic and chromogenic media

M. R. Naratama; I. Santoso

doi:10.1088/1742-6596/1442/1/012064

Non-fecal and fecal coliform tests of ready-to-eat food and drinks using fluorogenic and chromogenic media

M. R. Naratama, I. Santoso

Department of Biology

Research output: Contribution to journal › Conference article › peer-review

Abstract

The aim of this research was to assess the microbiological quality of ready-to-eat foods and drinks. Fifteen samples were enriched in buffered peptone water (BPW) before tested for the presence of coliforms using two chromogenic media (Chromocult Coliform Agar-Enhanced Selectivity (CCA-ES) and Harlequin E. coli agar (HEC)) and a fluorogenic medium (Readycult Coliform 100 (RC 100)) at 37°C. Results showed that all samples contained non-Escherichia. coli coliforms and 12 of them contained Escherichia coli. Twelve E. coli strains and 15 non-E. coli coliform isolates were isolated. Fecal coliform tests showed that the isolated E. coli from 12 samples were of fecal origin while only 7 out of 15 coliform isolates were fecal non-E. coli coliforms.

Original language	English
Article number	012064
Journal	Journal of Physics: Conference Series
Volume	1442
Issue number	1
DOIs	https://doi.org/10.1088/1742-6596/1442/1/012064
Publication status	Published - 29 Jan 2020
Event	Basic and Applied Sciences Interdisciplinary Conference 2017, BASIC 2017 - , Indonesia Duration: 18 Aug 2017 → 19 Aug 2017

Keywords

chromo-fluorogenic media
coliform test
ready to eat food

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10.1088/1742-6596/1442/1/012064

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title = "Non-fecal and fecal coliform tests of ready-to-eat food and drinks using fluorogenic and chromogenic media",

abstract = "The aim of this research was to assess the microbiological quality of ready-to-eat foods and drinks. Fifteen samples were enriched in buffered peptone water (BPW) before tested for the presence of coliforms using two chromogenic media (Chromocult Coliform Agar-Enhanced Selectivity (CCA-ES) and Harlequin E. coli agar (HEC)) and a fluorogenic medium (Readycult Coliform 100 (RC 100)) at 37°C. Results showed that all samples contained non-Escherichia. coli coliforms and 12 of them contained Escherichia coli. Twelve E. coli strains and 15 non-E. coli coliform isolates were isolated. Fecal coliform tests showed that the isolated E. coli from 12 samples were of fecal origin while only 7 out of 15 coliform isolates were fecal non-E. coli coliforms.",

keywords = "chromo-fluorogenic media, coliform test, ready to eat food",

author = "Naratama, {M. R.} and I. Santoso",

note = "Funding Information: Ac k n o wle d g e m e n ts The research was financially supported by Universitas Indonesia. Publisher Copyright: {\textcopyright} Published under licence by IOP Publishing Ltd.; Basic and Applied Sciences Interdisciplinary Conference 2017, BASIC 2017 ; Conference date: 18-08-2017 Through 19-08-2017",

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T1 - Non-fecal and fecal coliform tests of ready-to-eat food and drinks using fluorogenic and chromogenic media

AU - Naratama, M. R.

AU - Santoso, I.

N1 - Funding Information: Ac k n o wle d g e m e n ts The research was financially supported by Universitas Indonesia. Publisher Copyright: © Published under licence by IOP Publishing Ltd.

PY - 2020/1/29

Y1 - 2020/1/29

N2 - The aim of this research was to assess the microbiological quality of ready-to-eat foods and drinks. Fifteen samples were enriched in buffered peptone water (BPW) before tested for the presence of coliforms using two chromogenic media (Chromocult Coliform Agar-Enhanced Selectivity (CCA-ES) and Harlequin E. coli agar (HEC)) and a fluorogenic medium (Readycult Coliform 100 (RC 100)) at 37°C. Results showed that all samples contained non-Escherichia. coli coliforms and 12 of them contained Escherichia coli. Twelve E. coli strains and 15 non-E. coli coliform isolates were isolated. Fecal coliform tests showed that the isolated E. coli from 12 samples were of fecal origin while only 7 out of 15 coliform isolates were fecal non-E. coli coliforms.

AB - The aim of this research was to assess the microbiological quality of ready-to-eat foods and drinks. Fifteen samples were enriched in buffered peptone water (BPW) before tested for the presence of coliforms using two chromogenic media (Chromocult Coliform Agar-Enhanced Selectivity (CCA-ES) and Harlequin E. coli agar (HEC)) and a fluorogenic medium (Readycult Coliform 100 (RC 100)) at 37°C. Results showed that all samples contained non-Escherichia. coli coliforms and 12 of them contained Escherichia coli. Twelve E. coli strains and 15 non-E. coli coliform isolates were isolated. Fecal coliform tests showed that the isolated E. coli from 12 samples were of fecal origin while only 7 out of 15 coliform isolates were fecal non-E. coli coliforms.

KW - chromo-fluorogenic media

KW - coliform test

KW - ready to eat food

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DO - 10.1088/1742-6596/1442/1/012064

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SN - 1742-6588

VL - 1442

JO - Journal of Physics: Conference Series

JF - Journal of Physics: Conference Series

IS - 1

M1 - 012064

T2 - Basic and Applied Sciences Interdisciplinary Conference 2017, BASIC 2017

Y2 - 18 August 2017 through 19 August 2017

ER -

Non-fecal and fecal coliform tests of ready-to-eat food and drinks using fluorogenic and chromogenic media

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Keywords

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