Nimotuzumab iodine-131 conjugated with MnFe2O4 for potential dual-modality (MRI/SPECT) imaging nanoprobe

Amal Rezka Putra, Yoki Yulizar, Rien Ritawidya, Dewangga Oky Bagus Apriandanu, Sumandi Juliyanto, Ahsanal Fikri, Rizki Marcony Surya

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Manganese ferrite nanoparticle (MnFe2O4 NPs) has introduced a versatile platform to develop a multifunctional nanosystem for Magnetic Resonance Imaging (MRI) and Single-Photon Emission Computed Tomography (SPECT) modalities. Herein, we develop novel iodine-131-labeled-nimotuzumab conjugated with MnFe2O4 NPs as multimodalities of MRI and SPECT nanoprobe. The synthesis of MnFe2O4 NPs was mediated by Piper nigrum seed extract (PNE) consisting of alkaloids and terpenoids which play essential roles as a weak base source and stabilizing agent in the formation of MnFe2O4 NPs. anti-epidermal Growth Factor Receptor (EGFR) nimotuzumab was conjugated with MnFe2O4 NPs via a heterobifunctional PEG-linker. The radiolabeling of nimotuzumab/MnFe2O4 with iodine-131 was performed using the iodogen method. The vibrational, optical, magnetic, and morphological properties of MnFe2O4 NPs were characterized. MnFe2O4 NPs exhibited a spherical shape with an average particle size of 21 nm with a magnetization value of 23.4 emu/g. The vibrational and structural analyses verify that the MnFe2O4-nimotuzumab was conjugated using a linker. As a result, 131I-labeled-MnFe2O4-nimotuzumab NPs represents an excellent radiochemical purity of 99.32 ± 0.22 %, suggesting that radiopharmaceutical product generates a high imaging quality and unnecessary radiation. In this study, we offer a novel approach to design 131I-labeled-MnFe2O4-nimotuzumab NPs for potential dual-modality MRI and SPECT imaging nanoprobe.

Original languageEnglish
Article number116016
JournalMaterials Science and Engineering B: Solid-State Materials for Advanced Technology
Volume286
DOIs
Publication statusPublished - Dec 2022

Keywords

  • Iodine-131
  • Manganese ferrite nanoparticles
  • MRI/SPECT imaging
  • Nimotuzumab
  • Piper nigrum

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