New species of the genus metschnikowia isolated from flowers in indonesia, metschnikowia cibodasensis sp.nov

Wellyzar Sjamsuridzal, Ariyanti Oetari, Chiharu Nakashima, Atit Kanti, Rasti Saraswati, Yantyati Widyastuti, Katsuhiko Ando

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A novel species, Metschnikowia cibodasensis, is proposed to accommodate eight strains (ID030093T, ID03-0094, ID03-0095, ID03-0096, ID03-0097, ID03-0098, ID03-0099, and ID03-0109) isolated from flowers of Saurauia pendula, Berberis nepalensis, and Brunfelsia americana in Cibodas Botanical Garden, West Java, Indonesia. The type strain of M. cibodasensis is ID030093T (= NBRC 101693T =UICC Y-335T = BTCC-Y25T). The common features of M. cibodasensis are a spherical to ellipsoidopedunculate shaped ascus, which contains one or two needleshaped ascospores, and lyse at maturity. Asci generally develop directly from vegetative cells but sometimes from chlamydospores. The neighbor-joining tree based on the D1/D2 domain of nuclear large subunit (nLSU) ribosomal DNA sequences strongly supports that M. cibodasensis (eight strains) and its closest teleomorphic species, M. reukaufii, are different species by a 100% bootstrap value. The type strain of M. cibodasensis, ID03-0093T, differed from M. reukaufii NBRC 1679T by six nt (five substitutions and one deletion) in their D1/D2 region of nLSU rDNA, and by 18 nt (five deletions, four insertions, and nine substitutions) in their internal transcribed spacer regions of rDNA, respectively. Four strains representative of M. cibodasensis (ID03-0093T, ID03-0095, ID03-0096, and ID03-0099) showed a mol% G+C content of 44.05 ± 0.25%, whereas that of M. reukaufii NBRC 1679T was 41.3%. The low value of DNADNA homology (5-16%) in four strains of M. cibodasensis and M. reukaufii NBRC 1679T strongly supported that these strains represent a distinct species.

Original languageEnglish
Pages (from-to)905-912
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume23
Issue number7
DOIs
Publication statusPublished - 31 Jul 2013

Keywords

  • Ascomycetous yeast
  • Flowers
  • Metschnikowia cibodasensis
  • Taxonomy

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